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Ion Exchange Column
 
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This video demonstrates the use of a cation exchange column to convert a salt to an acid. This allows for the analysis of the total number of cations present in a given salt sample by titrating the resulting acid with a base
Views: 72959 crharrison
Principles of Ion Exchange Chromatography
 
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All our Principles and Methodology handbooks are available here: https://www.gelifesciences.com/handbooks Ion exchange chromatography is one of the most frequently used techniques for purification of biomolecules and separates the molecules according to differences in their net surface charge. This video describes the principles of the technique.
Ion exchange resin columns
 
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Views: 7005 RD L
Ion Exchange Chromatography
 
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Donate here: http://www.aklectures.com/donate.php Website video link: http://www.aklectures.com/lecture/ion-exchange-chromatography Facebook link: https://www.facebook.com/aklectures Website link: http://www.aklectures.com
Views: 138197 AK LECTURES
Quick guide to performing ion exchange chromatography
 
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An breif introduction to cation exchange columns. 1. Draining the equilibration buffer. 0m0s 2. Load and run the Amino Acid (AA) mixture. 1m37s 2. Load Citrate buffer to run AA mixture further into column. 4m24s 3. Summary diagram? 6m56s Note the video was recorded during the class and includes a practical demonstration and a written explanation (pen and paper). These two discussions were spliced together and each cover the same material.
Views: 23909 Mark Temple
LEARN AND GROW !! ION EXCHANGE PROCESS FOR WATER SOFTENING !
 
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On this channel you can get education and knowledge for general issues and topics
Views: 112712 LEARN AND GROW
Ion exchange
 
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The principle of ion exchange explained
Views: 9061 MetrohmTV
Ion Exchange Chromatography - Theory and Principle
 
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http://technologyinscience.blogspot.com/2013/07/an-insight-on-ion-exchange.html The principle of Ion Exchange chromatography separation is the reversible interaction of charged species with the ion exchange matrix. Ion Exchange Chromatography is of Two types: Cation Exchange and Anion Exchange.
Views: 147651 Bio-Resource
DEAE Sephacel Column:  Protein Purification via Ion Exchange
 
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Introduction to Ion Exchange Chromatography for Protein Purification.
Views: 8951 acr92651
Webinar: Tips for successful ion exchange chromatography
 
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www.gelifesciences.com/ProteinResearch Watch this 50-minute webinar and learn how to: • Optimize the resolution/purity of your target protein • Maximize protein recovery • Reduce hands-on time by optimizing your system’s configuration • Select the right ion exchange chromatography columns and resins to boost your results and efficiency
Anion Exchange Chromatography (GFP Purification part 2 of 6)
 
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The Purification process begins as the transfer tank of clarified lysate from the Recovery process is connected to the inlet pump on the Chromatography skid. The first Chromatography step in our GFP purification process is Anion-Exchange. At this point in the process, the pH of the clarified lysate is about eight-point-zero, which means that the protein is negatively charged. Because it is negatively charged, GFP will bind to the positively charged anion exchange resin. The pump draws the lysate from the vessel...past the first conductivity sensor and pressure sensor...and through the zero-point-four-five micron pre-filter. The pre-filter removes any residual cell debris or other particulates that may have contaminated the solution. If the pre-filter begins to clog, the pressure sensor at the inlet side of the filter will register a rising pressure...and the controller will signal the need for a filter change. After pre-filtering and before the column, the lysate passes through a flow meter... and an air sensor. Then, as the lysate passes over the resin beads, the negatively charged protein attaches to the positively charged beads. The solution leaving the column passes a UV optical density sensor, a conductivity sensor and a pH sensor. The optical density sensor's low readings confirm that the GFP is not in the solution, so the outlet valve sends the solution to waste.
Views: 16502 BioNetwork
IonExchange
 
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How ion exchange can be used to soften hard water.
Views: 150178 Clayton Spencer
ION EXCHANGE COLUMN
 
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Views: 8460 symecengineers
Basic for Operational and Column Design of Ion Exchange
 
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Its a basic understanding of operational and column design of ion exchange column... Enjoy the info... #credit to yana, Ain n zatul
Views: 239 Solihin Hamzah
Backwashing an Ion Exchange Column
 
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This video shows how to backwash an ion exchange column prior to regenerating the column.
Views: 1542 UAlbertaScience
Ion Exchange Column - Fixed Bed
 
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To learn more about ion exchange columns, visit the Visual Encyclopedia of Chemical Engineering: http://encyclopedia.che.engin.umich.edu/Pages/SeparationsChemical/IonExchangeColumns/IonExchangeColumns.html
Ion exchange chromatography | cation exchange chromatography and anion exchange chromatography
 
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This comment about the video lecture explains about ion exchange chromatography principle. It also explains the step-by-step process of ion exchange chromatography. That includes the the cation exchange chromatography and anion exchange chromatography. Ion exchange chromatography involves using positive and negative in charge ions to separate charged molecules from a mixture. In the positive this is a problem was in video. For more information, log on to- http://www.shomusbiology.com/ Get Shomu's Biology DVD set here- http://www.shomusbiology.com/dvd-store/ Download the study materials here- http://shomusbiology.com/bio-materials.html Remember Shomu’s Biology is created to spread the knowledge of life science and biology by sharing all this free biology lectures video and animation presented by Suman Bhattacharjee in YouTube. All these tutorials are brought to you for free. Please subscribe to our channel so that we can grow together. You can check for any of the following services from Shomu’s Biology- Buy Shomu’s Biology lecture DVD set- www.shomusbiology.com/dvd-store Shomu’s Biology assignment services – www.shomusbiology.com/assignment -help Join Online coaching for CSIR NET exam – www.shomusbiology.com/net-coaching We are social. Find us on different sites here- Our Website – www.shomusbiology.com Facebook page- https://www.facebook.com/ShomusBiology/ Twitter - https://twitter.com/shomusbiology SlideShare- www.slideshare.net/shomusbiology Google plus- https://plus.google.com/113648584982732129198 LinkedIn - https://www.linkedin.com/in/suman-bhattacharjee-2a051661 Youtube- https://www.youtube.com/user/TheFunsuman Thank you for watching
Views: 151718 Shomu's Biology
Ion Exchange chromatography, How to detect compound  that elutes from your column
 
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While washing of my compound from dowex resin, energy is released. This energy is coming from product released from the resin and can be felt and even measured as an elevation in temperature. In this case its even 25 degrees Celsius.
Views: 781 BoltYes
Ion Exchange Column
 
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Ion Exchange Column
Views: 20470 BCHKID1
Ion Exchange Column - Ion Exchange
 
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To learn more about ion exchange columns, visit the Visual Encyclopedia of Chemical Engineering: http://encyclopedia.che.engin.umich.edu/Pages/SeparationsChemical/IonExchangeColumns/IonExchangeColumns.html
Ion Exchange Chromatography Column Elution
 
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The following is a lecture given by Damien Terry at Texas A&M University for Biochemistry II Lab. It is a guide to column elution in ion exchange chromatography.
Introduction to Ion-exchange chromatography
 
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This video explains the fundamentals of ion exchange chromatography and demonstrates buffer selection for protein analysis. More information: https://www.agilent.com/en/products/liquid-chromatography/infinitylab-lc-workflow-solutions/infinitylab-bio-inert-lc-solutions/1260-infinity-ii-bio-inert-lc-system
Views: 52533 Agilent Technologies
Mod-01 Lec-38 Ion Exchange Processes
 
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Novel Separation Processes by Dr. Sirshendu De,Department of Chemical Engineering, IIT Kharagpur. For more details on NPTEL visit http://nptel.iitm.ac.in
Views: 3290 nptelhrd
Chromatography 101: An Introduction to Ion Exchange Chromatography
 
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For more information, visit http://www.bio-rad.com/yt/26/ngc. Shawn Anderson presents an introduction to ion exchange chromatography using Bio-Rad's chromatography instruments, columns, and media to demonstrate the technique. Shawn covers the basic principles of ion exchange chromatography and touches on applications for the separation of proteins and other biomolecules using a variety of anion and cation exchange media. Bio-Rad's Successful Chromatography Webinar series provides a great introduction to the different chromatography methods available for research applications. Most medium pressure chromatography columns work with Bio-Rad's NGC preparative chromatography system. The NGC is designed to rapidly automate the purification of biomolecules. The flexible, modular, and economical design makes the NGC systems the instruments of choice for method development and scale-up. NGC systems are available in six preplumbed, factory-tested configurations with two different flow rates. Each preconfigured system can be further customized and upgraded by adding valves, detectors, or pumps to meet specific application needs. Any system can be configured for operation at either high or low flow rate by simply changing the pump head on the system pump module. With the NGC system, a single hardware platform can be modified as needed to suit changing applications and scales. . http://www.bio-rad.com/evportal/destination/product?catID=MFCV7415&WT.mc_id=yt-pfd-ww-ngc-20130701-zytDmnoioJo We Are Bio-Rad. Our mission: To provide useful, high-quality products and services that advance scientific discovery and improve healthcare. At Bio-Rad, we are united behind this effort. These two objectives are the driving force behind every decision we make, from developing innovative ideas to building global solutions that help solve our customers' greatest challenges. Connect with Bio-Rad Online: Website: http://www.bio-rad.com/ LinkedIn: https://www.linkedin.com/company/1613226/ Facebook: https://www.facebook.com/biorad/ Twitter: https://twitter.com/BioRadLifeSci Instagram: @BioRadLabs Snapchat: @BioRadLabs
Views: 35919 Bio-Rad Laboratories
Demineralisation process (Deionization/Ion-exchange process) - Water Technology
 
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This video explains the demineralisation process in detail. ion-exchange process. Water softening/water purification method. Removal of ions/minerals from hard water. Subscribe for more videos !!!.
Views: 1362 Revathi Purushothaman
Ion Exchange Chromatography
 
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Author: Karson Self Explains the process of IEC.
Views: 19411 noonereeal
ion exchange (Rate of migration)
 
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Column operations
Views: 83 Phezo One
Ion Exchange Process
 
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This animation shows the process that happens during ion exchange
Views: 19170 Alonso Rocha Tapia
Anion Exchange Chromatography - separating haemoglobin and catalase
 
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This video demonstrates how to pour a DEAE-Sepharose column for the separation of the proteins haemoglobin and catalase. The video also shows the running of the column and the separation of the proteins.
Views: 15718 Nick Morris
A Brief Introduction to Packing, Loading and Running Chromatography Columns
 
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Professor Davis briefly reviews the anatomy of a chromatography column and how packing and loading a column can affect the quality of the subsequent separation.
Views: 116461 ChemSurvival
Unused- Graver Water Systems, Mixed Bed Vessel Ion Exchange Column Tank - stk# 46532003
 
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Unused- Graver Water Systems Approximate 4000 Gallon Mixed Bed Vessel Ion Exchange Column Tank. Manufactured by Kennedy Tank, 304/304L stainless steel, vertical. 120" Diameter x 60" straight side, 2:1 elliptical top and bottom. Internal rated 250 psi at -20 to 135 degrees F. Openings top 12" service inlet, 3" resin transfer inlet, side top 24" man way with a carbon steel davit arm, 1" NTP test connection, (2) 3" NTP grout filling, (2) 4" sight glass, 12" tangential service outlet, bottom (2) 3" resin transfer outlet, 1" NTP tell-tale drain. Mounted on 4 carbon steel I-Beam legs. National Board# 7980, serial# 9261-06A, built 2009.
Making hard water soft | Chemistry for All | The Fuse School
 
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Learn the basics about making hard water turn into soft water. What are the methods and techniques used to make that happen? Find out more in this video! This Open Educational Resource is free of charge, under a Creative Commons License: Attribution-NonCommercial CC BY-NC ( View License Deed: http://creativecommons.org/licenses/by-nc/4.0/ ). You are allowed to download the video for nonprofit, educational use. If you would like to modify the video, please contact us: [email protected] SUBSCRIBE to the Fuse School YouTube channel for many more educational videos. Our teachers and animators come together to make fun & easy-to-understand videos in Chemistry, Biology, Physics, Maths & ICT. This video is part of 'Chemistry for All' - a Chemistry Education project by our Charity Fuse Foundation - the organisation behind The Fuse School. These videos can be used in a flipped classroom model or as a revision aid. Find our other Chemistry videos here: https://www.youtube.com/playlist?list=PLW0gavSzhMlReKGMVfUt6YuNQsO0bqSMV Twitter: https://twitter.com/fuseSchool Access a deeper Learning Experience in the Fuse School platform and app: www.fuseschool.org Follow us: http://www.youtube.com/fuseschool Friend us: http://www.facebook.com/fuseschool
Ion Exchange Resin Packing
 
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Staff is working for the packing of cation exchange resin. 25Liter or 25Kg plastic bags sealed for loading with pallets to overseas customers.
Views: 670 ION EXCHANGE RESIN
Unused- Graver Water Systems, Mixed Bed Vessel Ion Exchange Column Tank - stk# 46532004
 
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Unused- Graver Water Systems Approximate 4000 Gallon Mixed Bed Vessel Ion Exchange Column Tank. Manufactured by Kennedy Tank, 304/304L stainless steel, vertical. 120" Diameter x 60" straight side, 2:1 elliptical top and bottom. Internal rated 250 psi at -20 to 135 degrees F. Openings top 12" service inlet, 3" resin transfer inlet, side top 24" man way with a carbon steel davit arm, 1" NTP test connection, (2) 3" NTP grout filling, (2) 4" sight glass, 12" tangential service outlet, bottom (2) 3" resin transfer outlet, 1" NTP tell-tale drain. Mounted on 4 carbon steel I-Beam legs. National Board# 7981, serial# 9261-06B, built 2009.
Unused- Graver Water Systems, Mixed Bed Vessel Ion Exchange Column Tank - stk# 46532002
 
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Unused- Graver Water Systems Approximate 4000 Gallon Mixed Bed Vessel Ion Exchange Column Tank. Manufactured by Kennedy Tank, 304/304L stainless steel, vertical. 120" Diameter x 60" straight side, 2:1 elliptical top and bottom. Internal rated 250 psi at -20 to 135 degrees F. Openings top 12" service inlet, 3" resin transfer inlet, side top 24" man way with a carbon steel davit arm, 1" NTP test connection, (2) 3" NTP grout filling, (2) 4" sight glass, 12" tangential service outlet, bottom (2) 3" resin transfer outlet, 1" NTP tell-tale drain. Mounted on 4 carbon steel I-Beam legs. National Board# 7984, serial# 9261-06E, built 2009.
High-Performance Anion-Exchange Chromatography (HPAE-PAD)
 
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http://www.dionex.com/en-us/products/columns/bio/carbohydrate/lp-73362.html High-Performance Anion-Exchange Chromatography (HPAE-PAD) enables faster and more accurate analysis or anionic analytes and carbohydrates without derivatization, including mono-, di-, tri-, oligo- and polysaccharides, sugar alcohols, and amino sugars. HPAE uses hydroxide-based eluents at high pH to produce anions from analytes that would not be anionic at neutral pH. Chromatography with these eluents is possible due the development of metal-free, polymer based chromatographic systems and pH stable (0 to 14) nonporous polymeric resins, in which small anion-exchange MicroBeads* carrying the anion-exchange functional groups are permanently attached electrostatically to a larger cation-exchange resin particle. The nonporous nature of the resin minimizes band-broadening and imparts highly effective separation of a wide variety of carbohydrates, including branched oligosaccharides. A range of different columns for carbohydrate separations from mono, di-, tri- to oligo- and polysaccharides is available under the Thermo Scientific Dionex CarboPac columns brand. http://www.dionex.com/en-us/products/columns/bio/carbohydrate/lp-73362.html
Views: 1534 Thermo Scientific
Basics of chromatography | Chemical processes | MCAT | Khan Academy
 
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Understand the basic principles of different kinds of chromatography: paper, thin layer, column, size-exchange, ion exchange, affinity, HPLC, and. By Angela Guerrero. . Created by Angela Guerrero. Watch the next lesson: https://www.khanacademy.org/test-prep/mcat/chemical-processes/separations-purifications/v/thin-layer-chromatography?utm_source=YT&utm_medium=Desc&utm_campaign=mcat Missed the previous lesson? https://www.khanacademy.org/test-prep/mcat/chemical-processes/separations-purifications/v/extractions?utm_source=YT&utm_medium=Desc&utm_campaign=mcat MCAT on Khan Academy: Go ahead and practice some passage-based questions! About Khan Academy: Khan Academy offers practice exercises, instructional videos, and a personalized learning dashboard that empower learners to study at their own pace in and outside of the classroom. We tackle math, science, computer programming, history, art history, economics, and more. Our math missions guide learners from kindergarten to calculus using state-of-the-art, adaptive technology that identifies strengths and learning gaps. We've also partnered with institutions like NASA, The Museum of Modern Art, The California Academy of Sciences, and MIT to offer specialized content. For free. For everyone. Forever. #YouCanLearnAnything Subscribe to Khan Academy’s MCAT channel: https://www.youtube.com/channel/UCDkK5wqSuwDlJ3_nl3rgdiQ?sub_confirmation=1 Subscribe to Khan Academy: https://www.youtube.com/subscription_center?add_user=khanacademy
Views: 575569 khanacademymedicine
Unused- Graver Water Systems, Cation Vessel Ion Exchange Column Tank - stock # 46532008
 
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Unused- Graver Water Systems Approximate 4700 Gallon Cation Vessel Ion Exchange Column Tank. Manufactured by Kennedy Tank, 316/316L stainless steel, vertical. 120" Diameter x 76" straight side, 2:1 elliptical top and bottom. Internal rated 250 psi at -20 to 135 degrees F. Openings top 12" service inlet, 4" resin transfer inlet, side top 24" man way with a carbon steel davit arm, 1" NTP test connection, (2) 3" NTP grout filling, (2) 4" sight glass, 12" tangential service outlet, bottom (2) 4" resin transfer outlet, 1" NTP tell-tale drain. Mounted on 4 carbon steel I-Beam legs. National Board# 7988, serial# 9261-07D, built 2009.
Unused- Graver Water Systems Approximate 470Cation Vessel Ion Exchange Column Tank - stck#46532010
 
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Unused- Graver Water Systems Approximate 4700 Gallon Cation Vessel Ion Exchange Column Tank. Manufactured by Kennedy Tank, 316/316L stainless steel, vertical. 120" Diameter x 76" straight side, 2:1 elliptical top and bottom. Internal rated 250 psi at -20 to 135 degrees F. Openings top 12" service inlet, 4" resin transfer inlet, side top 24" man way with a carbon steel davit arm, 1" NTP test connection, (2) 3" NTP grout filling, (2) 4" sight glass, 12" tangential service outlet, bottom (2) 4" resin transfer outlet, 1" NTP tell-tale drain. Mounted on 4 carbon steel I-Beam legs. National Board# 7986, serial# 9261-07B, built 2009.
Column Packing with Ca⁺⁺Pure-HA™ Hydroxyapatite Resin
 
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Bill Evans, Technical Service Specialist demonstrates packing a 10 cm ID pilot scale column to a bed height of 20 cm with Ca⁺⁺Pure-HA hydroxyapatite resin. For more information on this hydroxyapatite resin, please see our website: https://www.separations.us.tosohbioscience.com/Process_Media/id-7016/Ca%E2%81%BA%E2%81%BAPure-HA
Views: 4199 Tosoh Bioscience LLC
Column Chromatography Basics and Lab Procedure
 
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In this video I have discussed about what is Column Chromatography, what is its principle and lab procedure and How to perform Column Chromatography in lab manually step by step. First step is to choose the stationary phase based on sample. Second step is to pack the column, packing can be done by two method 1) Dry method 2) Wet method. Third step is to choose Mobile Phase Forth step is to load sample onto the packed column. Sample gets purified on the basis of polarity. Later we have also look in brief at isocratic elution and gradient elution. Please watch this video for more detail. If you like this video please like and share this video, and don’t forget to subscribe and click on bell icon for new videos. Subscribe: https://www.youtube.com/c/BaaYo?sub_confirmation=1 Related Videos: HPLC Chromatography: https://youtu.be/9P_SIiMheZk Thin Layer Chromatography : https://youtu.be/HFGRXL9Ihds Ion Exchange Chromatography : https://youtu.be/vMzs4NyVvuc Gas Chromatography: https://youtu.be/0KqaIKHzHE8 #BaaYo #ColumnChromatography
Views: 3906 BaaYo
Ion Exchange Chromatography (IEC): Introduction, Types of Resins and Mobile Phases
 
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Subject:Analytical Chemistry/Instrumentation Paper: Chromatographic techniques
Views: 51 Vidya-mitra

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