Home
Search results “Cytometric analysis of cell”
An introduction to flow cytometric analysis, Part I:  Cell proliferation analysis
 
45:36
In this 2-part series, we will give an overview of tools and techniques using Invitrogen reagents for flow cytometric analysis of cell proliferation, viability, vitality, and apoptosis. Part I covers cell proliferation.
An introduction to flow cytometric analysis, Part 2:  Cell viability and apoptosis analysis
 
52:13
In this webinar, we will discuss flow cytometric analysis of apoptosis and identification of dead cells using numerous assays for Membrane structure, Mitochondrial function, metabolism, caspase activity, membrane integrity, and DNA fragmentation.
Propidium Iodide Staining
 
03:32
( http://www.abnova.com ) - Propidium iodide (or PI) is an intercalating agent and a fluorescent molecule that can be used to stain DNA. PI is used as a DNA stain for both flow cytometry to evaluate cell viability or DNA content in cell cycle analysis and microscopy to visualize the nucleus and other DNA containing organelles. It can be used to differentiate necrotic, apoptotic and normal cells. More videos at Abnova http://www.abnova.com
Views: 30395 Abnova
Analysis of Cell Vitality and Viability Using Calcein-AM / PI
 
02:37
Fluorescent Detection of Metabolically-Active and Non-viable Cells using Cellometer Image Cytometry
Views: 957 Nexcelom Bioscience
Optimize your Flow Cytometry - Best Practices for Sample Preparation, Staining and Analysis
 
56:21
For more information, visit http://www.bio-rad.com/HuCAL-Flow In this webinar we cover best practice to prepare single cell suspensions from different sources to have a viable, contamination free sample. We also include staining tips and explain how dead cell and doublet removal can improve your data. https://www.bio-rad-antibodies.com/custom-monoclonal-antibody-generation.html?WT.mc_id=sm-abd-ww-abdserotec-vyt-20160824-oeqiCa3nEwc We Are Bio-Rad. Our mission: To provide useful, high-quality products and services that advance scientific discovery and improve healthcare. At Bio-Rad, we are united behind this effort. These two objectives are the driving force behind every decision we make, from developing innovative ideas to building global solutions that help solve our customers' greatest challenges. Connect with Bio-Rad Antibodies Online: Website: https://www.bio-rad-antibodies.com/ LinkedIn: https://www.linkedin.com/showcase/bio-rad-antibodies/ Facebook: https://www.facebook.com/BioRadAntibodies/ Twitter: https://twitter.com/BioRadAbs Pinterest: https://www.pinterest.com/BioRadAbs/ Instagram: @BioRadLabs Snapchat: @BioRadLabs
Views: 14786 Bio-Rad Laboratories
Flow Cytometry
 
12:10
This cell biology tutorial explains about flow cytometry procedure and cell count analysis. Web- http://shomusbiology.com/ Download the study materials here- http://shomusbiology.com/bio-materials.html Remember Shomu’s Biology is created to spread the knowledge of life science and biology by sharing all this free biology lectures video and animation presented by Suman Bhattacharjee in YouTube. All these tutorials are brought to you for free. Please subscribe to our channel so that we can grow together. You can check for any of the following services from Shomu’s Biology- Buy Shomu’s Biology lecture DVD set- www.shomusbiology.com/dvd-store Shomu’s Biology assignment services – www.shomusbiology.com/assignment -help Join Online coaching for CSIR NET exam – www.shomusbiology.com/net-coaching We are social. Find us on different sites here- Our Website – www.shomusbiology.com Facebook page- https://www.facebook.com/ShomusBiology/ Twitter - https://twitter.com/shomusbiology SlideShare- www.slideshare.net/shomusbiology Google plus- https://plus.google.com/113648584982732129198 LinkedIn - https://www.linkedin.com/in/suman-bhattacharjee-2a051661 Youtube- https://www.youtube.com/user/TheFunsuman Thank you for watching This video explains the flow cytometry techniques and its importance in cell biology.
Views: 194674 Shomu's Biology
Fluorescence activated cell sorting (FACS)
 
09:12
This lecture explains about Fluorescence activated cell sorting better known as FACS. This is a technique for cell sorting using fluorescent probe. For more information, log on to- http://www.shomusbiology.com/ Get Shomu's Biology DVD set here- http://www.shomusbiology.com/dvd-store/ Download the study materials here- http://shomusbiology.com/bio-materials.html Remember Shomu’s Biology is created to spread the knowledge of life science and biology by sharing all this free biology lectures video and animation presented by Suman Bhattacharjee in YouTube. All these tutorials are brought to you for free. Please subscribe to our channel so that we can grow together. You can check for any of the following services from Shomu’s Biology- Buy Shomu’s Biology lecture DVD set- www.shomusbiology.com/dvd-store Shomu’s Biology assignment services – www.shomusbiology.com/assignment -help Join Online coaching for CSIR NET exam – www.shomusbiology.com/net-coaching We are social. Find us on different sites here- Our Website – www.shomusbiology.com Facebook page- https://www.facebook.com/ShomusBiology/ Twitter - https://twitter.com/shomusbiology SlideShare- www.slideshare.net/shomusbiology Google plus- https://plus.google.com/113648584982732129198 LinkedIn - https://www.linkedin.com/in/suman-bhattacharjee-2a051661 Youtube- https://www.youtube.com/user/TheFunsuman Thank you for watching
Views: 54696 Shomu's Biology
Flow Cytometry
 
16:26
This lecture explains about flow cytometry technique in details. Flow cytometry is a technology that is used to analyse the physical and chemical characteristics of particles in a fluid as it passes through at least one laser. This video lecture will help you to understand the mechanism of flow cytometry to find out number of cells and their shapes. For more information, log on to- http://www.shomusbiology.com/ Get Shomu's Biology DVD set here- http://www.shomusbiology.com/dvd-store/ Download the study materials here- http://shomusbiology.com/bio-materials.html Remember Shomu’s Biology is created to spread the knowledge of life science and biology by sharing all this free biology lectures video and animation presented by Suman Bhattacharjee in YouTube. All these tutorials are brought to you for free. Please subscribe to our channel so that we can grow together. You can check for any of the following services from Shomu’s Biology- Buy Shomu’s Biology lecture DVD set- www.shomusbiology.com/dvd-store Shomu’s Biology assignment services – www.shomusbiology.com/assignment -help Join Online coaching for CSIR NET exam – www.shomusbiology.com/net-coaching We are social. Find us on different sites here- Our Website – www.shomusbiology.com Facebook page- https://www.facebook.com/ShomusBiology/ Twitter - https://twitter.com/shomusbiology SlideShare- www.slideshare.net/shomusbiology Google plus- https://plus.google.com/113648584982732129198 LinkedIn - https://www.linkedin.com/in/suman-bhattacharjee-2a051661 Youtube- https://www.youtube.com/user/TheFunsuman Thank you for watching the video lecture on Flow Cytometry.
Views: 88849 Shomu's Biology
Flow Cytometry - Cell Cycle
 
02:07
( http://www.abnova.com ) - This AbVideo focuses on the flow cytometry analysis for a population of cell distribution at different cell cycle stages. Flow cytometry is a technique for measuring and examining microscopic particles in a flow system, which delivers particles singly by passing through an electronic detection apparatus. More videos at Abnova http://www.abnova.com
Views: 23253 Abnova
Advanced Topics in Cell Sorting
 
53:24
For more info, visit http://www.bio-rad.com/yt/4/S3 In this webinar, Dan Fox of Propel Labs explores the principles of cell sorting including the theory of droplet cell sorting, how to assess cell sorter performance, and how sorting speed influences results. Dan also discusses the S3™ Cell Sorter’s unique sorting modes, the sort mode process and logic, and how the S3 Sorter simplifies the science of cell sorting. The first truly walk-up cell sorter, the S3 delivers an easy-to-use, affordable solution for busy scientists. The S3 Cell Sorter is an automated benchtop cell sorter featuring either one or two lasers and up to four fluorescence detectors plus forward- and side-scatter detectors. The S3 Cell Sorter is ideal for sorting cells expressing fluorescent proteins such as eGFP, DsRed, and mCherry or cells bearing fluorescent markers such as labeled antibodies. Features and Benefits • Simple instrument setup — automated drop delay calculation and droplet break-off monitoring enables precise 1–4 color sorting with minimal training • Compact footprint — only 70 × 65 × 65 cm (2.3 × 2.1 × 2.1 ft) including onboard fluidics and temperature control system • Reduced carryover — dual-position loading stage ensures the sample line remains clean between sorting runs • 2-way cell sorting — sort two different defined populations at the same time • High speed and high purity — sort cells fast while maintaining high sensitivity and purity • Intuitive interface — user-friendly ProSort™ software enables effortless instrument control and sort logic definition Presenter: Dan Fox Director of Research and Development Propel Labs, Fort Collins, Colorado Dan Fox has over a decade of experience in designing high-speed cell sorters, and holds numerous patents on flow cytometry and sorting technology. Dan is one of the principal designers who developed Bio-Rad’s S3 Cell Sorter. http://www.bio-rad.com/en-us/category/flow-cytometry?WT.mc_id=yt-GXD-ww-s3-20141006-N-kHOL6sJdM We Are Bio-Rad. Our mission: To provide useful, high-quality products and services that advance scientific discovery and improve healthcare. At Bio-Rad, we are united behind this effort. These two objectives are the driving force behind every decision we make, from developing innovative ideas to building global solutions that help solve our customers' greatest challenges. Connect with Bio-Rad Online: Website: http://www.bio-rad.com/ LinkedIn: https://www.linkedin.com/company/1613226/ Facebook: https://www.facebook.com/biorad/ Twitter: https://twitter.com/BioRadCellBio Instagram: @BioRadLabs Snapchat: @BioRadLabs
Views: 15272 Bio-Rad Laboratories
Flow Cytometry Animation
 
02:38
Brief introduction to flow cytometry from Cell Signaling Technology (CST). Visit 👉https://www.cellsignal.com/flow for more information on validated products and optimized protocols for flow cytometry. Transcript: Cell processes are controlled by complex and dynamic intracellular signaling networks. Surface receptors pass information into the cell by modifying cytoplasmic proteins. These modifications include phosphorylation,. methylation, acetylation, which control a broad range of cellular functions, including growth, development, and apoptosis. Disruption of signaling networks often leads to disease. To understand signaling processes and normal and pathological conditions, identification and quantification of signaling proteins is necessary. Flow cytometry is ideal for such a task. Flow cytometry is a laser based technology widely used to phenotype cells using antibodies directed against surface markers. It can also allow simultaneous analysis of multiple intracellular targets using antibodies that detects and signaling proteins, their post-translational modifications, epigenetic modifications, and downstream transcription factors. The first step of intracellular flow cytometry is fixation of the cells using formaldehyde, which immobilizes proteins and preserves transient signaling events. Cells are then permeabilized with methanol or detergent, allowing antibodies to access to the intracellular space. Optimal fixation and permeabilization conditions depend on the location and biochemistry of the target epitopes. It should be carefully considered to ensure successful antigen detection, especially when staining surface- and intracellular proteins simultaneously. Since multiple signaling proteins and surface antigens can be identified using fluorescently distinct antibodies, flow cytometry can readily characterize signaling profiles at the single-cell level within phenotypically distinct populations. This allows the characterization of the relationship between intracellular targets and enables detection of signaling events from rare-cell populations within a heterogeneous sample. Multiplex analysis of these protein targets saves time and resources while producing data-rich results, making flow cytometry an invaluable tool to unravel the complexities of cell signaling.
Nicole Weit - Advanced flow cytometric analysis of human T cell memory subsets
 
58:25
Watch on LabRoots at http://labroots.com/webinar/id/169 T-cells form an integral part of the human immune system by fighting off infection and eliminating transformed cells. Over the last decades numerous T-cells subsets with different functions and abilities have been identified (Farber et al. Nat Rev Immunol 2014). The wide spread use of monoclonal antibodies and multi-color flow cytometry allows for the routine identification of these subsets based on their immune-phenotype (Chattopadhyay et al. Cytometry 2012). However, the increasingly detailed understanding and the dynamic nature of different T-cell subsets also results in a dramatic increase in the complexity of the antibody panels used. Here, we will demonstrate the identification of naïve, memory and effector T cells at various stages of differentiation, but also the detection of rare populations such as stem cell like memory T cells and different Treg subpopulations using a 13-color marker combination that allows for the identification of T cell subtypes according to classical and more recent characterization criteria. We will outline the panel design rational taking into account expression patterns as well as fluorochrome characteristics. The applied gating strategies will be discussed.
Views: 4423 LabRoots
DNA content cell cycle analysis using flow cytometry
 
42:04
In this webinar we’ll discuss the methods & materials used to determine cell cycle by measuring DNA content, the selection of DNA dyes for live cell & fixed cell analysis, and Tips & tricks for consistent results.
Detect apoptosis using Flow cytometry analysis- CSIR NET
 
05:27
This video explain how the technique of flow cytometry is used to detect for the presence of apoptotic cells in a given cell population.
Views: 234 BioMagica
Flow Cytometry (Verbsky)
 
56:02
James Verbsky, MD, PhD describes the uses of flow cytometry for the diagnosis of primary immunodeficiency. Held on August 11, 2017.
Views: 2294 ACAAICOLA
Flow Cytometric Approach to Probiotic Cell Counting and Analysis
 
39:24
Flow Cytometric Approach to Probiotic Cell Counting and Analysis Presented by: Dharlene Tundo - Quality Control, Senior Microbiologist, VitaQuest International Speaker Biography: Dharlene Tundo obtained her Bachelor of Science in Molecular Biology from Montclair State University and has over ten years of diverse experience in research and pharmaceutical industry. Dharlene contributed to the successful launch of two oncology drugs, Cyramza and Portrazza, at Lilly Pharmaceutical. Her research interest is focused on the study of Lactobacillus and Bifidobacterium species. She is dedicated to develop a method to analyze and obtain cell count differentiation on viable and non-viable bacterial cells for the manufacture of probiotic supplements, using the flow cytometric approach. Webinar: Flow Cytometric Approach to Probiotic Cell Counting and Analysis Abstract: The complexity of the manufacturing process of probiotic supplements, along with increased demand and strict criteria for quality, potency and safety, in accordance to FDA regulations, necessitates that manufacturers continue to improve production processes that increase productivity and efficiency. Flow Cytometry is a viable option for a non-culture based method for enumerating cells for probiotics manufacture. Using the CytoFLEX flow cytometer with its ability to resolve small particles down to 200nm, it was possible to reliably count bacteria as small as 0.5μm. This analysis allowed to compare and contrast culture-based and flow cytometry based workflows for probiotic cell enumeration. The results obtained in this study indicate that flow cytometry is applicable for probiotic cell count assay; both dyes, SYTO9 and PI were able to identify viable and non-viable cells, providing the analyst additional information on the status of the cultures. Learning objectives: The importance of gating and threshold settings strategy Comparison between traditional probiotic cell counts versus a new approach using flow cytometry method Bacterial cell staining process Sponsored by: Beckman Coulter Life Sciences Earn PACE Credits: 1. Make sure you’re a registered member of LabRoots: https://www.labroots.com/ms/webinar/flow-cytometric-approach-probiotic-cell-counting-analysis 2. Watch the webinar on YouTube or on the LabRoots Website: https://www.labroots.com/ms/webinar/flow-cytometric-approach-probiotic-cell-counting-analysis 3. Click Here to get your PACE credits 2/15/2020: http://www.labroots.com/credit/pace-credits/2696/third-party LabRoots on Social: Facebook: https://www.facebook.com/LabRootsInc Twitter: https://twitter.com/LabRoots LinkedIn: https://www.linkedin.com/company/labroots Instagram: https://www.instagram.com/labrootsinc Pinterest: https://www.pinterest.com/labroots/ SnapChat: labroots_inc
Views: 177 LabRoots
How To Do Compensation in BD Accuri C6
 
05:32
Fluorescent Dyes: FITC and TRITC Excitation: 488nm Emission: FL1 (FITC) and FL2 (TRITC). Spillover calculation analysis using Thermofisher Spectrum Viewer. Sample Tubes: 1) Unstained Tube 2) FITC Control Tube 3) TRITC Control Tube 4) Sample of Interest (FITC + TRITC).
Views: 1914 Bio Technologies
Fluorescence-activated cell sorting (FACS)
 
12:10
Fluorescence-activated cell sorting (FACS) is a specialized type of flow cytometry. It provides a method for sorting a heterogeneous mixture of biological cells into two or more containers, one cell at a time, based upon the specific light scattering and fluorescent characteristics of each cell. It is a useful scientific instrument as it provides fast, objective and quantitative recording of fluorescent signals from individual cells as well as physical separation of cells of particular interest. The acronym FACS is trademarked and owned by Becton, Dickinson and Company.[21] Among the large majority of researchers who use this technology for sorting or analysis, this term has become generic in common usage, much like xerox or kleenex. The first cell sorter was invented by Mack Fulwyler in 1965, using the Coulter principle, a relatively difficult technique that is no longer used in modern instruments. The technique was expanded by Len Herzenberg, who was responsible for coining the term FACS.[22] Herzenberg won the Kyoto Prize in 2006 for his seminal work in flow cytometry. The cell suspension is entrained in the center of a narrow, rapidly flowing stream of liquid. The flow is arranged so that there is a large separation between cells relative to their diameter. A vibrating mechanism causes the stream of cells to break into individual droplets. The system is adjusted so that there is a low probability of more than one cell per droplet. Just before the stream breaks into droplets, the flow passes through a fluorescence measuring station where the fluorescent character of interest of each cell is measured. An electrical charging ring is placed just at the point where the stream breaks into droplets. A charge is placed on the ring based on the immediately prior fluorescence intensity measurement, and the opposite charge is trapped on the droplet as it breaks from the stream. The charged droplets then fall through an electrostatic deflection system that diverts droplets into containers based upon their charge. In some systems, the charge is applied directly to the stream, and the droplet breaking off retains charge of the same sign as the stream. The stream is then returned to neutral after the droplet breaks off.
Views: 155831 Athir Ch
The Basic FACS About ALDEFLUOR™ - A Guide To Successful Flow Cytometry Analysis
 
06:29
The ALDEFLUOR™ fluorescent reagent system has supported over 200 publications to date. This fast, antibody-free assay detects more than 80 types of stem, progenitor, and cancer precursor cells by measuring their activity levels of aldehyde dehydrogenase (ALDH) using a flow cytometer. This video guide demonstrates the correct use of the flow cytometer for analyzing ALDEFLUOR™-stained ALDH bright cells. For more information visit http://www.stemcell.com Subscribe to STEMCELL Technologies on YouTube at https://www.youtube.com/STEMCELLTechnologies Follow STEMCELL Technologies on Twitter at https://twitter.com/stem_cells Like STEMCELL Technologies on Facebook at https://www.facebook.com/stemcelltechnologies
Views: 39365 STEMCELLTechnologies
Flow Cytometry Animation
 
04:36
This animation on flow cytometry will introduce you to the flow cytometry experimental technique.
Views: 244897 mitedustar
Introduction to FACS Data Analysis
 
03:48
This video is to show how the FACS Data looks like. and what to select. Basic introduction to data analysis. Single Color Experiment. Fluorescent Dye: Turbo-RFP Excitation Wavelength: 561nm Emission Filter : 585/29.
Views: 7671 Bio Technologies
Simplified Benchtop Multicolor Flow Cytometry for Immune and Cancer Cell Analyses
 
50:46
Multicolor flow cytometry can dramatically increase the capability of cell analysis. However, traditional multicolor flow cytometers have posed challenges in choosing fluorescent dyes, managing compensation, and analyzing results. These challenges have required specific expertise and exclusive dedication of user resources, as well as significant consumption of costly reagents and difficult-to-obtain patient samples, such as blood and cerebrospinal fluid cells. Participants in this webinar will discuss the use of microcapillary flow cytometry systems to accelerate benchtop multicolor flow analyses for differentiation and characterization of animal-model and patient-derived lymphocytes. These include immune cells derived from mouse rheumatoid arthritis models as well as multiple sclerosis patients.
Views: 8371 GENNews
Flow Cytometry Tutorials: Doublet Discrimination
 
06:04
Learn to exclude doublets from your Flow Cytometry data analysis. By the end of this tutorial, you should be able to understand: What doublets and clumps are? Why do we need to gate out doublets and clumps? How to gate out doublets? And finally, different strategies to exclude doublets. Credit: Conceptualized and created by Javid Mohammed, Cincinnati, OH
Views: 55429 Flow Cytometry Network
Trends in Multiparameter Flow Cytometry in Hematology - Dr. Brent Wood
 
50:02
Flow cytometry is increasingly utilized as primary tool for the diagnosis of hematopoietic neoplasia in a clinical laboratory setting. The use of increased numbers of simultaneous fluorochromes offers the following potential advantages: increased accuracy of population identification, the ability to make better use of small specimens where cell number is a limiting factor, the processing of fewer tubes with the consequent reduction in reagents, technologist time and instrument time, the more efficient analysis of large numbers of cells which is important for the detection of small cell populations, and ultimately improved standardization. Brent Wood, associate professor of the hematology division in the department of laboratory medicine at the University of Washington discusses practical issues surrounding the implementation of high-level multicolor flow cytometry in a clinical laboratory setting and illustration of the power of the technology. Dr. Brent Wood
Views: 20726 UW Video
Basics of flow cytometry, Part I:  Gating and data analysis
 
56:07
This webinar covers the basic components of a flow cytometer, how to interpret a dye excitation/emission spectrum, how data is displayed, basic gating demonstration, and common statistics and terminology used in flow cytometry.
Data analysis and applications of Flow Cytometry
 
26:45
Subject:Biophysics Paper: Medical Physics
Views: 342 Vidya-mitra
Multicolor Panel Building in Flow Cytometry
 
56:05
For more information, visit https://www.bio-rad-antibodies.com/multicolor-webinar Multicolor flow cytometry is the analysis of multiple fluorescent parameters in one sample. Building large flow cytometry panels can be daunting because each additional fluorophore you add to your panel has the potential to influence another fluorophore. Join our webinar to learn which fluorophores are compatible with each other, how they interact and can affect your staining and how to obtain optimal resolution of signal. Furthermore, we will show you how dump channels, fluorophore brightness, antigen density, marker expression patterns and instrument configuration can all contribute to improving your panel design. This webinar is recommended for both novices and researchers with some experience of flow cytometry. Speaker Biography: Presented by: Dr Sebastian Hedlund, Flow Cytometry Specialist at Bio-Rad Laboratories Sebastian obtained his degree from the Institute of Technology, Linköping University, Sweden, followed by an MSc in Medical Biology from the same institute. He then moved to Freiburg, Germany where he obtained his PhD in Immunology and Molecular Biology at the Max Planck Institute of Immunobiology and Epigenetics. The focus of his thesis was the role of microRNA in innate immunity and immunopathology. He then left academia and started work as a dedicated flow cytometry specialist, before joining Bio-Rad in 2016 as the flow cytometry specialist for Scandinavia, Eastern Europe, Israel, and South Africa. Visit us on the web to learn more: https://www.bio-rad-antibodies.com/multicolor-panel-building-webinar.html We Are Bio-Rad. Our mission: To provide useful, high-quality products and services that advance scientific discovery and improve healthcare. At Bio-Rad, we are united behind this effort. These two objectives are the driving force behind every decision we make, from developing innovative ideas to building global solutions that help solve our customers' greatest challenges. Connect with Bio-Rad Online: Website: http://www.bio-rad.com/ LinkedIn: https://www.linkedin.com/company/1613226/ Facebook: https://www.facebook.com/biorad/ Twitter: https://twitter.com/BioRadLifeSci Instagram: @BioRadLabs Snapchat: @BioRadLabs
Views: 4729 Bio-Rad Laboratories
Basics of flow cytometry, Part II: Compensation
 
49:41
This webinar includes an overview of fluorochromes for flow cytometry, the principle of compensation, performing compensation, the types of controls recommended and their use, basic strategies for designing a flow experiment, and data presentation.
Multiparametric Flow Cytometry Analysis of Cell-mediated Immunity in Clinical Trials
 
06:53
Click here to register for free and to view the entire webinar: http://xtalks.com/Flow-Cytometry-Cell-mediated-Immununity.ashx?utm_source=Youtube&utm_medium=Teaser&utm_campaign=XTO863_Caprion_ImmuneCarta_MedicaGo Speakers: Jean-Francois Poulin, PhD, MBA, Senior Principal Scientist and Business Development Liaison, Caprion/IMMUNECARTA Services Stéphane Pillet, PhD, Scientifique immunologie / Scientist Immunology, Medicago Inc. Influenza infections are endemic in several regions of the world. The World Health Organization estimates that between 250,000 - 500,000 people die annually due to seasonal influenza viral infection, including 36 000 deaths in the USA alone. In the past century, the world has witnessed at least 4 influenza pandemics responsible for millions of deaths on a global scale. Estimates from the most recent pandemic (H1N1, 2009-2010) place the world-wide toll between 123,000 and 203,000 deaths, highlighting the need for faster and scalable approaches to developing and deploying new, more effective influenza vaccines. In this scientific webinar, Caprion and Medicago will describe how the production of Virus-Like Particle (VLP)-based vaccines using plant-based transient expression technology has particular advantages for accelerating both the development of new vaccines and the scaling-up of production for deployment in affected areas. Vaccine lots, which exactly match the specific strain(s) in circulation can be generated within a month of strain identification. This is a significant improvement compared to more traditional fertilized chicken egg-based platforms, which require half a year or more for viral strain production. Plant-based VLP technology is rapid, scalable and relatively low-cost, with the added safety advantage that the VLP contains no genetic material, rendering it non-infectious and unable to replicate. Hemagglutination inhibition (HI) antibody titers, the only universally accepted immune correlate of protection against influenza, does not necessarily translate into protection, and clinical benefit may be short-lived, particularly in the elderly. Recent studies in animal models have suggested that in situations where antibodies fail to confer protection, cell-mediated immunity may be able to act alone, suggesting that some shortcomings currently associated with influenza vaccines might be surmounted via use of novel vaccines which elicit CD4 and CD8 T cell responses. Therefore, in the current clinical immune monitoring study, the specificity and magnitude of CD4 and CD8 T cell response were measured following immunization. The data presented will show how multiparametric flow cytometry and related analytical tools allow monitoring the diversity and distribution of Influenza-specific cellular subsets both at the phenotypic and functional level in the context of a clinical trial. The results of these major studies were published in the recent issue of Clinical Immunology, entitled “Influenza virus-like particle vaccines made in Nicotiana enthamiana elicit durable, poly-functional and cross-reactive T cell responses to influenza HA antigens”.
Views: 418 Xtalks Webinars
The Principle of Flow Cytometry and FACS (1- Flow Cytometry)
 
10:29
This video is an explanation of Flow Cytometry, it contains a full explanation about flow cytometer and data obtaned from it.
BD Accuri C6, flow cytometry made easy. Analyzing one cell at a time
 
09:40
In this episode, I present to you, maybe the best entry level flow cytometer in the market, BD Accuri C6. I would say a must-have equipment for every lab focused on studying cells. Also, a brief introduction to flow cytometry and a step by step guide to measuring cell viability by this versatile technique.
Views: 642 Bio Sciences_4all
Flow Cytometry - Double Stain
 
02:15
( http://www.abnova.com ) - Step by step of double staining for flow cytometry analysis of cells is presented. Flow cytometry is a technique for measuring and examining microscopic particles in a flow system, which delivers particles singly by passing through an electronic detection apparatus. More videos at Abnova http://www.abnova.com
Views: 8395 Abnova
Running a Basic 2 color Flow Cytometry Experiment in BD FACS Diva
 
27:31
This video describes how to set up an experiment in FACS Diva version 8.0 on an LSR II flow cytometer.
Expanding Cell Analysis in Cancer Research with Imaging Flow Cytometry
 
01:11:29
For a PDF of the slides, click here: http://bit.ly/18kpMCM Imaging flow cytometry combines the speed, sensitivity, and phenotyping capabilities of flow cytometry with the detailed imagery and functional insights of microscopy. This unique combination has enabled a broad range of applications for cancer cell analysis that would be impossible using either technique alone. In this webinar, Dr. Andrew Filby of The London Research Institute will describe the recent development of a novel method for reporting the cell cycle position and division history of asynchronously dividing cells. This method allowed detailed analysis of cell cycle inhibitor function down to the mitotic stage across multiple division rounds. John Hazin of the German Cancer Research Center will describe how imaging flow cytometry expanded understanding of cell cycle and cell adhesion molecules in cancer biology in his laboratory. Hazin and his colleagues identified a subset of cells in which the transmembrane cell-adhesion molecule L1CAM (L1) forms polarized membrane domains. They further investigated the co-localization of other proteins with L1, as well as nuclear translocation of an intracellular L1 fragment believed to participate in signaling functions. These examples demonstrate the practical use and the power of imaging flow cytometry.
Views: 3359 GENNews
Webinar: Rare Cell Analysis in Flow Cytometry
 
59:32
Join Frank Hardung and his webinar on rare cell analysis in flow cytometry, its general approaches, and proper controls. MACS Academy: https://www.miltenyibiotec.com/resources/macs-academy.html Miltenyi Biotec is a global provider of products and services that advance biomedical research and cellular therapy. Our innovative tools support research at every level, from basic research to translational research to clinical application. This integrated portfolio enables scientists and clinicians to obtain, analyze, and utilize the cell. Our technologies cover techniques of sample preparation, cell isolation, cell sorting, flow cytometry, cell culture, molecular analysis, and preclinical imaging. Our more than 25 years of expertise spans research areas including immunology, stem cell biology, neuroscience, and cancer, and clinical research areas like hematology, graft engineering, and apheresis. In our commitment to the scientific community, we also offer comprehensive scientific support, consultation, and expert training. Today, Miltenyi Biotec has more than 2,000 employees in 25 countries – all dedicated to helping researchers and clinicians around the world make a greater impact on science and health.
Views: 2164 Miltenyi Biotec
Single-cell Profiling with Mass Cytometry
 
01:19:23
Mass cytometry, or CyTOF, is a variation of flow cytometry in which antibodies are labeled with heavy metal ion tags rather than fluorochromes. Readout is by time-of-flight mass spectrometry. This allows for the combination of many more antibodies (over 30) for phenotypic profiling and functional analysis of single cells, without significant overlap between channels. Mass cytometry has now been applied to various research areas including basic immunology, neuroscience, immuno-oncology, drug discovery, and stem cell research. While mass cytometry offers a powerful and highly multiplexed method for profiling single cells, there are various technical considerations for researchers wanting to perform a mass cytometry assay for the first time, or at a more advanced level. These steps include experimental planning, sample and reagent preparation, validation, quality control, to data analysis. In this webinar, you will gain insights into how you can get the most out of multiplex single-cell profiling with mass cytometry from a leading expert.
Webinar: Tumor microenvironment - Tools for TIL isolation and flow cytometry analysis
 
23:20
In this webinar Dr. César Evaristo will describe best practices for isolation of tumor infiltrating leukocytes (TILs) and present an optimized workflow for faster and improved flow analysis. Webinar attendees will learn about innovative tools and solutions to increase quality of data, reduce experimental time, and ease scale-up during TIL analysis using flow cytometry. They will find out about optimized workflows for the isolation and flow cytometric phenotyping of mouse TILs, and methods to reduce biases during TIL analysis. Recent advances in immuno-oncology research have demonstrated the critical role of immune cells in cancer therapy. A deep understanding of the interactions and phenotypes of immune and tumor cells within the tumor microenvironment is therefore key to developing future immune-based cancer therapy strategies. However, dissociation of solid tumor samples prior to their use in downstream applications, such as immunomagnetic enrichment, flow cytometry, and single-cell sequencing, can be challenging. In addition, tumor cells often cause background signal and introduce bias to the analysis of immune cells, further complicating TIL research. Dr. César Evaristo, who received his doctorate from the University of Paris Descartes, will present the webinar. After seven years as a research scientist in the United States and Germany, he joined Miltenyi Biotec in 2016 as a team coordinator of the Tumor Immunology Group. He focuses on immuno-oncology preclinical research, target discovery, as well as oncology-related product development. Find more Miltenyi Biotec webinars at https://www.miltenyibiotec.com/macs-academy ABOUT MILTENYI BIOTEC Miltenyi Biotec is a global provider of products and services that advance biomedical research and cellular therapy. The company’s innovative tools support research at every level, from basic research to translational research to clinical application. This integrated portfolio enables scientists and clinicians to obtain, analyze, and utilize the cell. Miltenyi Biotec’s technologies cover techniques of sample preparation, cell isolation, cell sorting, flow cytometry, cell culture, molecular analysis, and preclinical imaging. Their more than 25 years of expertise spans research areas including immunology, stem cell biology, neuroscience, and cancer, and clinical research areas like hematology, graft engineering, and apheresis. In their commitment to the scientific community, Miltenyi Biotec also offers comprehensive scientific support, consultation, and expert training. Today, Miltenyi Biotec has more than 2,000 employees in 25 countries – all dedicated to helping researchers and clinicians around the world make a greater impact on science and health.
Views: 168 Miltenyi Biotec
Flow Cytometry Webinar: From sample preparation to experimental design
 
48:19
This advanced class in flow cytometry discusses sample preparation and the process of experimental design to ensure a successful flow cytometry experiment. Webinar topics include: 1. Cell source and preparation 2. Sample treatments 3. Controls and maximising signal 4. Choosing and using antibodies This video was produced by Innova Biosciences which is now fully integrated with Expedeon Ltd. - To find out more about our range of products for flow cytometry applications, please visit: https://bit.ly/2OiQEAs -To learn more about this powerful technique, feel free to download our guide: https://bit.ly/2IllhQo
Views: 47218 Expedeon
Multiparameter Cell Cycle Analysis
 
35:07
Most cell cycle assays amount to counting cells and/or measuring DNA content and deconvolving the resulting histogram into G1, S, G2+M, or G1, S, G2, and M. Considerable effort has been put to correlating DNA content with RNA, protein, or specific epitopes to determine or discover additional compartments. Here, we'll focus on flow cytometric analysis of DNA and specific epitopes. In recent efforts, using DNA peak to mark anaphase/cytokinetic populations in a 6 parameter analysis (DNA-area, DNA-peak, Light Scatter, cyclin A2, cyclin B1, and phospho-S10-histone H3), we identify 15 compartments with more than 4 additional compartments representing apoptotic cells. Rational approaches to fixation and optimized staining protocols were worked out years ago. We have examined many experimental variations on the basic protocols without uncovering any major improvements that result in higher quality data. We are working currently on a “washless” staining assay, relying on a single high dilution to minimize background staining and relying on acoustic focusing to render the sample analyzable over a short period. The results are striking. The S/N is equivalent to a fully washed sample; the minimized handling results in better cell recovery, and improved recovery results in better definition of cell cycle compartments with low cell numbers. Additionally, the “washless” assay provides significant labor savings. For research use only. Not for use in diagnostic procedures.
Views: 69 LabRoots
Mastering Phospho Flow: Optimizing Immune Cell Activation Assays
 
29:47
Combined analysis of immune phenotyping and rare signaling events can be performed using flow cytometry, but presents technical challenges. Topics covered in this workshop include: •Guidance on combining surface markers with phospho-specific intracellular targets; •How small protocol changes can impact flow cytometry results; •Examples of optimization using antibody panels for T cell activation and innate immunity; •Discussion on use of fix-and-perm kits Presented by Chris Manning, Group Leader of Flow Cytometry at Cell Signaling Technology. When you think about what you're usually doing by live-cell flow, you've got access to 370 CD markers and about 1400 proteins on the plasma membrane surface. Of course, if you fix and permeabilize those cells, now you're talking about an order of magnitude more proteins you can detect and over 400,000 modifications on those proteins as measured by mass spectrometry. That's really where the biology of these cells is driven. These are the readouts that let you understand what's going on in the cell. What is the cell doing? Where is the cell headed in terms of differentiation or activity? 👉Subscribe: http://youtube.com/user/cellsignaldotcom?sub_confirmation=8 👉CST Protocols and Troubleshooting: http://cellsignal.com/protocols 👉Get in touch with a CST scientist: http://cellsignal.com/support 👉About CST: Cell Signaling Technology (CST) is a private, family-owned company, founded by scientists and dedicated to providing high-quality research tools to the biomedical research community. Our employees operate worldwide from our U.S. headquarters in Massachusetts, and our offices in the Netherlands, China, and Japan. http://cellsignal.com/about #FlowCytometry #PhosphoFlow
DNA Cell Cycle Analysis with Multicycle in FCS Express
 
53:47
Learn how to use the Multicycle DNA Analysis tool in FCS Express as well as details on how modeling DNA data with Multicycle works. Kevin Becker, from Phoenix Flow Systems, discusses analysis of DNA Cell Cycle while the team at De Novo Software shows you how multicycle easily fits into your analysis routine directly in FCS Express. Often when trying to analyze DNA histograms for cell cycle or DNA analysis, the use of simple region markers on the histogram is not adequate. Sometimes it is necessary to use a powerful mathematical model to remove the debris component and deconvolute the multiple peaks in the histogram. The MultiCycle AV DNA analysis plug-in for FCS Express addresses this problem in a seamless manner. The mathematical model of Dean and Jett, the gold standard, is used by the MultiCycle AV plug-in. In addition, sophisticated debris subtraction models developed over the past decade by leaders in the field of cell cycle analysis, Rabinovitch & Bagwell, are applied to the debris component. This results in accurate, simple to understand cell cycle results from even the most complicated DNA histogram.
Views: 999 DeNovoSoftware
Introduction to Flow Cytometry in R
 
01:17:25
Instructor: Ryan Brinkman
Views: 1010 Bioinformatics DotCa
Anti-human TIGIT, clone A15153G, for flow cytometry, cell separation, and functional assay
 
05:02
BioLegend’s new antibody against human TIGIT, clone A15153G, is an excellent reagent for examining the expression and function of human TIGIT. This poster video reviews application data of this antibody tested in flow cytometry, cell separation, cell depletion, and CD3 stimulation assays. Clone A15153G is an excellent research tool for immunology and cancer researchers interested in the function of TIGIT.
Views: 271 BioLegend
Flow Cytometry - Single Stain
 
01:38
( http://www.abnova.com ) - This AbVideo exemplifies the procedure of single staining for flow cytometry analysis of cells. Flow cytometry is a technique for measuring and examining microscopic particles in a flow system, which delivers particles singly by passing through an electronic detection apparatus. More videos at Abnova http://www.abnova.com
Views: 4957 Abnova
Webinar: Analysis of Intracellular Signalling using Flow Cytometry
 
48:30
Join Federica Villanova and her webinar on the analysis of intracellular signalling using flow cytometry. Learn more at http://www.miltenyibiotec.com/macs-academy Miltenyi Biotec is a global provider of products and services that advance biomedical research and cellular therapy. Our innovative tools support research at every level, from basic research to translational research to clinical application. This integrated portfolio enables scientists and clinicians to obtain, analyze, and utilize the cell. Our technologies cover techniques of sample preparation, cell isolation, cell sorting, flow cytometry, cell culture, molecular analysis, and preclinical imaging. Our more than 25 years of expertise spans research areas including immunology, stem cell biology, neuroscience, and cancer, and clinical research areas like hematology, graft engineering, and apheresis. In our commitment to the scientific community, we also offer comprehensive scientific support, consultation, and expert training. Today, Miltenyi Biotec has more than 2,000 employees in 25 countries – all dedicated to helping researchers and clinicians around the world make a greater impact on science and health.
Views: 1199 Miltenyi Biotec
Flow Cytometry
 
01:33
Views: 1154 Larry Huang
Webinar - Relax and Go with the Flow: Demystifying Multi-Parameter Flow Cytometry
 
01:12:23
More Flow Cytometry training webinars: https://www.rndsystems.com/product-highlights/flow-cytometry-training-webinars Watch part two here: https://youtu.be/XiOVPqlJb_w Flow cytometry is a powerful technique that allows researchers to examine multiple proteins on cell populations using fluorescently labeled antibodies. Multi-parameter, fluidic-based flow cytometry has been applied to multiple fields of study ranging from basic to clinical immunology, cancer biology, neuroscience, stem cell research, and drug discovery. In order to maximize the quality of results obtained using this technology, researchers should be familiar with the basics of flow cytometry when setting up an experiment. In this series of seminars, we will discuss the necessary tools to design, run, and analyze a multi-parameter flow cytometric experiment. In this introductory level webinar, you will learn the basics of how flow cytometry works including how to optimally set up and run a multi-parameter experiment. We will highlight the following topics: - Spectral overlap and how compensation is used to correct this issue - How the surface density of cellular antigens influences fluorochrome choice - Basic data analysis To illustrate these points, we will feature a short video on how to run a basic multicolor flow experiment on human natural Tregs, using techniques which can be applied to any field of study. https://www.bio-techne.com https://www.rndsystems.com https://www.novusbio.com https://www.tocris.com https://www.proteinsimple.com https://www.acdbio.com

Uk cover letter structure
Job cover letter salutation line
Lanxess newsletter formats
Country club cover letters
Chilis employment application form