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An introduction to flow cytometric analysis, Part I:  Cell proliferation analysis
 
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In this 2-part series, we will give an overview of tools and techniques using Invitrogen reagents for flow cytometric analysis of cell proliferation, viability, vitality, and apoptosis. Part I covers cell proliferation.
An introduction to flow cytometric analysis, Part 2:  Cell viability and apoptosis analysis
 
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In this webinar, we will discuss flow cytometric analysis of apoptosis and identification of dead cells using numerous assays for Membrane structure, Mitochondrial function, metabolism, caspase activity, membrane integrity, and DNA fragmentation.
Flow Cytometry - Cell Cycle
 
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( http://www.abnova.com ) - This AbVideo focuses on the flow cytometry analysis for a population of cell distribution at different cell cycle stages. Flow cytometry is a technique for measuring and examining microscopic particles in a flow system, which delivers particles singly by passing through an electronic detection apparatus. More videos at Abnova http://www.abnova.com
Views: 23752 Abnova
DNA content cell cycle analysis using flow cytometry
 
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In this webinar we’ll discuss the methods & materials used to determine cell cycle by measuring DNA content, the selection of DNA dyes for live cell & fixed cell analysis, and Tips & tricks for consistent results.
Detect apoptosis using Flow cytometry analysis- CSIR NET
 
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This video explain how the technique of flow cytometry is used to detect for the presence of apoptotic cells in a given cell population.
Views: 572 BioMagica
Flow Cytometric Approach to Probiotic Cell Counting and Analysis
 
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Flow Cytometric Approach to Probiotic Cell Counting and Analysis Presented by: Dharlene Tundo - Quality Control, Senior Microbiologist, VitaQuest International Speaker Biography: Dharlene Tundo obtained her Bachelor of Science in Molecular Biology from Montclair State University and has over ten years of diverse experience in research and pharmaceutical industry. Dharlene contributed to the successful launch of two oncology drugs, Cyramza and Portrazza, at Lilly Pharmaceutical. Her research interest is focused on the study of Lactobacillus and Bifidobacterium species. She is dedicated to develop a method to analyze and obtain cell count differentiation on viable and non-viable bacterial cells for the manufacture of probiotic supplements, using the flow cytometric approach. Webinar: Flow Cytometric Approach to Probiotic Cell Counting and Analysis Abstract: The complexity of the manufacturing process of probiotic supplements, along with increased demand and strict criteria for quality, potency and safety, in accordance to FDA regulations, necessitates that manufacturers continue to improve production processes that increase productivity and efficiency. Flow Cytometry is a viable option for a non-culture based method for enumerating cells for probiotics manufacture. Using the CytoFLEX flow cytometer with its ability to resolve small particles down to 200nm, it was possible to reliably count bacteria as small as 0.5μm. This analysis allowed to compare and contrast culture-based and flow cytometry based workflows for probiotic cell enumeration. The results obtained in this study indicate that flow cytometry is applicable for probiotic cell count assay; both dyes, SYTO9 and PI were able to identify viable and non-viable cells, providing the analyst additional information on the status of the cultures. Learning objectives: The importance of gating and threshold settings strategy Comparison between traditional probiotic cell counts versus a new approach using flow cytometry method Bacterial cell staining process Sponsored by: Beckman Coulter Life Sciences Earn PACE Credits: 1. Make sure you’re a registered member of LabRoots: https://www.labroots.com/ms/webinar/flow-cytometric-approach-probiotic-cell-counting-analysis 2. Watch the webinar on YouTube or on the LabRoots Website: https://www.labroots.com/ms/webinar/flow-cytometric-approach-probiotic-cell-counting-analysis 3. Click Here to get your PACE credits 2/15/2020: http://www.labroots.com/credit/pace-credits/2696/third-party LabRoots on Social: Facebook: https://www.facebook.com/LabRootsInc Twitter: https://twitter.com/LabRoots LinkedIn: https://www.linkedin.com/company/labroots Instagram: https://www.instagram.com/labrootsinc Pinterest: https://www.pinterest.com/labroots/ SnapChat: labroots_inc
Views: 243 LabRoots
Flow Cytometry Animation
 
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Brief introduction to flow cytometry from Cell Signaling Technology (CST). Visit 👉https://www.cellsignal.com/flow for more information on validated products and optimized protocols for flow cytometry. Transcript: Cell processes are controlled by complex and dynamic intracellular signaling networks. Surface receptors pass information into the cell by modifying cytoplasmic proteins. These modifications include phosphorylation,. methylation, acetylation, which control a broad range of cellular functions, including growth, development, and apoptosis. Disruption of signaling networks often leads to disease. To understand signaling processes and normal and pathological conditions, identification and quantification of signaling proteins is necessary. Flow cytometry is ideal for such a task. Flow cytometry is a laser based technology widely used to phenotype cells using antibodies directed against surface markers. It can also allow simultaneous analysis of multiple intracellular targets using antibodies that detects and signaling proteins, their post-translational modifications, epigenetic modifications, and downstream transcription factors. The first step of intracellular flow cytometry is fixation of the cells using formaldehyde, which immobilizes proteins and preserves transient signaling events. Cells are then permeabilized with methanol or detergent, allowing antibodies to access to the intracellular space. Optimal fixation and permeabilization conditions depend on the location and biochemistry of the target epitopes. It should be carefully considered to ensure successful antigen detection, especially when staining surface- and intracellular proteins simultaneously. Since multiple signaling proteins and surface antigens can be identified using fluorescently distinct antibodies, flow cytometry can readily characterize signaling profiles at the single-cell level within phenotypically distinct populations. This allows the characterization of the relationship between intracellular targets and enables detection of signaling events from rare-cell populations within a heterogeneous sample. Multiplex analysis of these protein targets saves time and resources while producing data-rich results, making flow cytometry an invaluable tool to unravel the complexities of cell signaling.
Fluorescence activated cell sorting (FACS)
 
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This lecture explains about Fluorescence activated cell sorting better known as FACS. This is a technique for cell sorting using fluorescent probe. For more information, log on to- http://www.shomusbiology.com/ Get Shomu's Biology DVD set here- http://www.shomusbiology.com/dvd-store/ Download the study materials here- http://shomusbiology.com/bio-materials.html Remember Shomu’s Biology is created to spread the knowledge of life science and biology by sharing all this free biology lectures video and animation presented by Suman Bhattacharjee in YouTube. All these tutorials are brought to you for free. Please subscribe to our channel so that we can grow together. You can check for any of the following services from Shomu’s Biology- Buy Shomu’s Biology lecture DVD set- www.shomusbiology.com/dvd-store Shomu’s Biology assignment services – www.shomusbiology.com/assignment -help Join Online coaching for CSIR NET exam – www.shomusbiology.com/net-coaching We are social. Find us on different sites here- Our Website – www.shomusbiology.com Facebook page- https://www.facebook.com/ShomusBiology/ Twitter - https://twitter.com/shomusbiology SlideShare- www.slideshare.net/shomusbiology Google plus- https://plus.google.com/113648584982732129198 LinkedIn - https://www.linkedin.com/in/suman-bhattacharjee-2a051661 Youtube- https://www.youtube.com/user/TheFunsuman Thank you for watching
Views: 59001 Shomu's Biology
Expanding Cell Analysis in Cancer Research with Imaging Flow Cytometry
 
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For a PDF of the slides, click here: http://bit.ly/18kpMCM Imaging flow cytometry combines the speed, sensitivity, and phenotyping capabilities of flow cytometry with the detailed imagery and functional insights of microscopy. This unique combination has enabled a broad range of applications for cancer cell analysis that would be impossible using either technique alone. In this webinar, Dr. Andrew Filby of The London Research Institute will describe the recent development of a novel method for reporting the cell cycle position and division history of asynchronously dividing cells. This method allowed detailed analysis of cell cycle inhibitor function down to the mitotic stage across multiple division rounds. John Hazin of the German Cancer Research Center will describe how imaging flow cytometry expanded understanding of cell cycle and cell adhesion molecules in cancer biology in his laboratory. Hazin and his colleagues identified a subset of cells in which the transmembrane cell-adhesion molecule L1CAM (L1) forms polarized membrane domains. They further investigated the co-localization of other proteins with L1, as well as nuclear translocation of an intracellular L1 fragment believed to participate in signaling functions. These examples demonstrate the practical use and the power of imaging flow cytometry.
Views: 3376 GENNews
Using Flow Cytometry to Quantify Immune Response and Cell-Mediated Cytotoxicity
 
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Immunotherapy drugs and anti-viral treatments often work by stimulating the body’s immune system. Assays are needed to understand the mechanisms of cell-mediated killing to help improve these treatments. Simple reagents, such as MTT, LDH, ATP, will generate live/dead data as general indicators of cellular metabolism but cannot distinguish between cell types. ELISAs often require cell lysis. 51Cr-release assays have regulatory issues and safety concerns when working with isotopes. Flow cytometry assays offer significant advantages over other methods by providing single-cell analysis and multiplexing of reagents. For example, ICT’s Basic and Total Cytotoxicity Kits are able to differentiate target cells when cultured with immune effector cells, and concurrently quantify live, necrotic, and apoptotic cells. In this presentation, we will discuss how to quantify cell-mediated cytotoxicity using flow cytometry.
Flow Cytometry
 
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This lecture explains about flow cytometry technique in details. Flow cytometry is a technology that is used to analyse the physical and chemical characteristics of particles in a fluid as it passes through at least one laser. This video lecture will help you to understand the mechanism of flow cytometry to find out number of cells and their shapes. For more information, log on to- http://www.shomusbiology.com/ Get Shomu's Biology DVD set here- http://www.shomusbiology.com/dvd-store/ Download the study materials here- http://shomusbiology.com/bio-materials.html Remember Shomu’s Biology is created to spread the knowledge of life science and biology by sharing all this free biology lectures video and animation presented by Suman Bhattacharjee in YouTube. All these tutorials are brought to you for free. Please subscribe to our channel so that we can grow together. You can check for any of the following services from Shomu’s Biology- Buy Shomu’s Biology lecture DVD set- www.shomusbiology.com/dvd-store Shomu’s Biology assignment services – www.shomusbiology.com/assignment -help Join Online coaching for CSIR NET exam – www.shomusbiology.com/net-coaching We are social. Find us on different sites here- Our Website – www.shomusbiology.com Facebook page- https://www.facebook.com/ShomusBiology/ Twitter - https://twitter.com/shomusbiology SlideShare- www.slideshare.net/shomusbiology Google plus- https://plus.google.com/113648584982732129198 LinkedIn - https://www.linkedin.com/in/suman-bhattacharjee-2a051661 Youtube- https://www.youtube.com/user/TheFunsuman Thank you for watching the video lecture on Flow Cytometry.
Views: 98575 Shomu's Biology
Cell Cycle Analysis by Flow Cytometry (Webinar)
 
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DNA content analysis using flow cytometry is a powerful tool that can determine the effect of treatments on cell cycle and ploidy in particular relevance to tumours. However, how do you determine which DNA-binding dye to use? How do you accurately differentiate the different phases in the cell cycle in your population of cells? This webinar will teach the methods for cell cycle analysis with the following topics: During this webinar, we will cover: -Phases of a typical mammalian cell cycle -Characteristics of Nucleic Acid Stains -How to choose the right fluorochromes and properly prepare your cells -DNA ploidy & tumours Presenter: Stephanie Ting, M. Eng Field Application Scientist – SE Asia, ANZ, HK&Macau, India, ACEA Biosciences Inc.
Introduction to FACS Data Analysis
 
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This video is to show how the FACS Data looks like. and what to select. Basic introduction to data analysis. Single Color Experiment. Fluorescent Dye: Turbo-RFP Excitation Wavelength: 561nm Emission Filter : 585/29.
Views: 9308 Bio Technologies
Nicole Weit - Advanced flow cytometric analysis of human T cell memory subsets
 
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Watch on LabRoots at http://labroots.com/webinar/id/169 T-cells form an integral part of the human immune system by fighting off infection and eliminating transformed cells. Over the last decades numerous T-cells subsets with different functions and abilities have been identified (Farber et al. Nat Rev Immunol 2014). The wide spread use of monoclonal antibodies and multi-color flow cytometry allows for the routine identification of these subsets based on their immune-phenotype (Chattopadhyay et al. Cytometry 2012). However, the increasingly detailed understanding and the dynamic nature of different T-cell subsets also results in a dramatic increase in the complexity of the antibody panels used. Here, we will demonstrate the identification of naïve, memory and effector T cells at various stages of differentiation, but also the detection of rare populations such as stem cell like memory T cells and different Treg subpopulations using a 13-color marker combination that allows for the identification of T cell subtypes according to classical and more recent characterization criteria. We will outline the panel design rational taking into account expression patterns as well as fluorochrome characteristics. The applied gating strategies will be discussed.
Views: 4676 LabRoots
Multiparametric Flow Cytometry Analysis of Cell-mediated Immunity in Clinical Trials
 
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Click here to register for free and to view the entire webinar: http://xtalks.com/Flow-Cytometry-Cell-mediated-Immununity.ashx?utm_source=Youtube&utm_medium=Teaser&utm_campaign=XTO863_Caprion_ImmuneCarta_MedicaGo Speakers: Jean-Francois Poulin, PhD, MBA, Senior Principal Scientist and Business Development Liaison, Caprion/IMMUNECARTA Services Stéphane Pillet, PhD, Scientifique immunologie / Scientist Immunology, Medicago Inc. Influenza infections are endemic in several regions of the world. The World Health Organization estimates that between 250,000 - 500,000 people die annually due to seasonal influenza viral infection, including 36 000 deaths in the USA alone. In the past century, the world has witnessed at least 4 influenza pandemics responsible for millions of deaths on a global scale. Estimates from the most recent pandemic (H1N1, 2009-2010) place the world-wide toll between 123,000 and 203,000 deaths, highlighting the need for faster and scalable approaches to developing and deploying new, more effective influenza vaccines. In this scientific webinar, Caprion and Medicago will describe how the production of Virus-Like Particle (VLP)-based vaccines using plant-based transient expression technology has particular advantages for accelerating both the development of new vaccines and the scaling-up of production for deployment in affected areas. Vaccine lots, which exactly match the specific strain(s) in circulation can be generated within a month of strain identification. This is a significant improvement compared to more traditional fertilized chicken egg-based platforms, which require half a year or more for viral strain production. Plant-based VLP technology is rapid, scalable and relatively low-cost, with the added safety advantage that the VLP contains no genetic material, rendering it non-infectious and unable to replicate. Hemagglutination inhibition (HI) antibody titers, the only universally accepted immune correlate of protection against influenza, does not necessarily translate into protection, and clinical benefit may be short-lived, particularly in the elderly. Recent studies in animal models have suggested that in situations where antibodies fail to confer protection, cell-mediated immunity may be able to act alone, suggesting that some shortcomings currently associated with influenza vaccines might be surmounted via use of novel vaccines which elicit CD4 and CD8 T cell responses. Therefore, in the current clinical immune monitoring study, the specificity and magnitude of CD4 and CD8 T cell response were measured following immunization. The data presented will show how multiparametric flow cytometry and related analytical tools allow monitoring the diversity and distribution of Influenza-specific cellular subsets both at the phenotypic and functional level in the context of a clinical trial. The results of these major studies were published in the recent issue of Clinical Immunology, entitled “Influenza virus-like particle vaccines made in Nicotiana enthamiana elicit durable, poly-functional and cross-reactive T cell responses to influenza HA antigens”.
Views: 433 Xtalks Webinars
Tech Spotlight: High-Throughput Flow Cytometry on Adherent Cells
 
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Suspend-C is a chemical discovered by KAUST researchers making high-throughput flow cytometry on adherent cells a reality. It offers a simple alternative to cumbersome protocols currently in use for preparing adherent cell lines for medium to high-throughput screening using flow cytometers. Routinely used protocols on adherent cells include various wash and centrifugation steps, which are not only time consuming and low-throughput but also result in loss of cells (in our experience, up to 85% in four washes). This method allows high-throughput flow cytometry on adherent cells in 96- and 384-well plates. Adherent cells can be lifted without removing media, eliminating wash steps and maintaining 100% of cells in the well. This invention is part of KAUST's technology commercialization program that seeks to stimulate development and commercial use of KAUST-developed technologies. For more information: http://buff.ly/1irz82N
Views: 7005 KAUST Innovation
Optimize your Flow Cytometry - Best Practices for Sample Preparation, Staining and Analysis
 
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For more information, visit http://www.bio-rad.com/HuCAL-Flow In this webinar we cover best practice to prepare single cell suspensions from different sources to have a viable, contamination free sample. We also include staining tips and explain how dead cell and doublet removal can improve your data. https://www.bio-rad-antibodies.com/custom-monoclonal-antibody-generation.html?WT.mc_id=sm-abd-ww-abdserotec-vyt-20160824-oeqiCa3nEwc We Are Bio-Rad. Our mission: To provide useful, high-quality products and services that advance scientific discovery and improve healthcare. At Bio-Rad, we are united behind this effort. These two objectives are the driving force behind every decision we make, from developing innovative ideas to building global solutions that help solve our customers' greatest challenges. Connect with Bio-Rad Antibodies Online: Website: https://www.bio-rad-antibodies.com/ LinkedIn: https://www.linkedin.com/showcase/bio-rad-antibodies/ Facebook: https://www.facebook.com/BioRadAntibodies/ Twitter: https://twitter.com/BioRadAbs Pinterest: https://www.pinterest.com/BioRadAbs/ Instagram: @BioRadLabs Snapchat: @BioRadLabs
Views: 15765 Bio-Rad Laboratories
Flow Cytometry
 
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This cell biology tutorial explains about flow cytometry procedure and cell count analysis. Web- http://shomusbiology.com/ Download the study materials here- http://shomusbiology.com/bio-materials.html Remember Shomu’s Biology is created to spread the knowledge of life science and biology by sharing all this free biology lectures video and animation presented by Suman Bhattacharjee in YouTube. All these tutorials are brought to you for free. Please subscribe to our channel so that we can grow together. You can check for any of the following services from Shomu’s Biology- Buy Shomu’s Biology lecture DVD set- www.shomusbiology.com/dvd-store Shomu’s Biology assignment services – www.shomusbiology.com/assignment -help Join Online coaching for CSIR NET exam – www.shomusbiology.com/net-coaching We are social. Find us on different sites here- Our Website – www.shomusbiology.com Facebook page- https://www.facebook.com/ShomusBiology/ Twitter - https://twitter.com/shomusbiology SlideShare- www.slideshare.net/shomusbiology Google plus- https://plus.google.com/113648584982732129198 LinkedIn - https://www.linkedin.com/in/suman-bhattacharjee-2a051661 Youtube- https://www.youtube.com/user/TheFunsuman Thank you for watching This video explains the flow cytometry techniques and its importance in cell biology.
Views: 202969 Shomu's Biology
Propidium Iodide Staining
 
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( http://www.abnova.com ) - Propidium iodide (or PI) is an intercalating agent and a fluorescent molecule that can be used to stain DNA. PI is used as a DNA stain for both flow cytometry to evaluate cell viability or DNA content in cell cycle analysis and microscopy to visualize the nucleus and other DNA containing organelles. It can be used to differentiate necrotic, apoptotic and normal cells. More videos at Abnova http://www.abnova.com
Views: 31273 Abnova
Webinar: Exosome Analysis Using a Multiplex Bead-Based Flow Cytometry Assay
 
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Join Dr. Stefan Wild for his webinar on exosome analysis as he explains exosome detection by flow cytometry and introduces the MACSplex Exosome Assay. Find more MACS Academy webinars at https://www.miltenyibiotec.com/macs-academy Miltenyi Biotec is a global provider of products and services that advance biomedical research and cellular therapy. Our innovative tools support research at every level, from basic research to translational research to clinical application. This integrated portfolio enables scientists and clinicians to obtain, analyze, and utilize the cell. Our technologies cover techniques of sample preparation, cell isolation, cell sorting, flow cytometry, cell culture, molecular analysis, and preclinical imaging. Our more than 25 years of expertise spans research areas including immunology, stem cell biology, neuroscience, and cancer, and clinical research areas like hematology, graft engineering, and apheresis. In our commitment to the scientific community, we also offer comprehensive scientific support, consultation, and expert training. Today, Miltenyi Biotec has more than 2,000 employees in 25 countries – all dedicated to helping researchers and clinicians around the world make a greater impact on science and health.
Views: 4917 Miltenyi Biotec
Flow Cytometry (Verbsky)
 
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James Verbsky, MD, PhD describes the uses of flow cytometry for the diagnosis of primary immunodeficiency. Held on August 11, 2017.
Views: 2935 ACAAICOLA
Fluorescence-activated cell sorting (FACS)
 
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Fluorescence-activated cell sorting (FACS) is a specialized type of flow cytometry. It provides a method for sorting a heterogeneous mixture of biological cells into two or more containers, one cell at a time, based upon the specific light scattering and fluorescent characteristics of each cell. It is a useful scientific instrument as it provides fast, objective and quantitative recording of fluorescent signals from individual cells as well as physical separation of cells of particular interest. The acronym FACS is trademarked and owned by Becton, Dickinson and Company.[21] Among the large majority of researchers who use this technology for sorting or analysis, this term has become generic in common usage, much like xerox or kleenex. The first cell sorter was invented by Mack Fulwyler in 1965, using the Coulter principle, a relatively difficult technique that is no longer used in modern instruments. The technique was expanded by Len Herzenberg, who was responsible for coining the term FACS.[22] Herzenberg won the Kyoto Prize in 2006 for his seminal work in flow cytometry. The cell suspension is entrained in the center of a narrow, rapidly flowing stream of liquid. The flow is arranged so that there is a large separation between cells relative to their diameter. A vibrating mechanism causes the stream of cells to break into individual droplets. The system is adjusted so that there is a low probability of more than one cell per droplet. Just before the stream breaks into droplets, the flow passes through a fluorescence measuring station where the fluorescent character of interest of each cell is measured. An electrical charging ring is placed just at the point where the stream breaks into droplets. A charge is placed on the ring based on the immediately prior fluorescence intensity measurement, and the opposite charge is trapped on the droplet as it breaks from the stream. The charged droplets then fall through an electrostatic deflection system that diverts droplets into containers based upon their charge. In some systems, the charge is applied directly to the stream, and the droplet breaking off retains charge of the same sign as the stream. The stream is then returned to neutral after the droplet breaks off.
Views: 160445 Athir Ch
Flow Cytometry Animation
 
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This animation on flow cytometry will introduce you to the flow cytometry experimental technique.
Views: 266918 mitedustar
Simplified Benchtop Multicolor Flow Cytometry for Immune and Cancer Cell Analyses
 
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Multicolor flow cytometry can dramatically increase the capability of cell analysis. However, traditional multicolor flow cytometers have posed challenges in choosing fluorescent dyes, managing compensation, and analyzing results. These challenges have required specific expertise and exclusive dedication of user resources, as well as significant consumption of costly reagents and difficult-to-obtain patient samples, such as blood and cerebrospinal fluid cells. Participants in this webinar will discuss the use of microcapillary flow cytometry systems to accelerate benchtop multicolor flow analyses for differentiation and characterization of animal-model and patient-derived lymphocytes. These include immune cells derived from mouse rheumatoid arthritis models as well as multiple sclerosis patients.
Views: 8478 GENNews
The Basic FACS About ALDEFLUOR™ - A Guide To Successful Flow Cytometry Analysis
 
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The ALDEFLUOR™ fluorescent reagent system has supported over 200 publications to date. This fast, antibody-free assay detects more than 80 types of stem, progenitor, and cancer precursor cells by measuring their activity levels of aldehyde dehydrogenase (ALDH) using a flow cytometer. This video guide demonstrates the correct use of the flow cytometer for analyzing ALDEFLUOR™-stained ALDH bright cells. For more information visit http://www.stemcell.com Subscribe to STEMCELL Technologies on YouTube at https://www.youtube.com/STEMCELLTechnologies Follow STEMCELL Technologies on Twitter at https://twitter.com/stem_cells Like STEMCELL Technologies on Facebook at https://www.facebook.com/stemcelltechnologies
Views: 39765 STEMCELLTechnologies
Webinar: Tumor microenvironment - Tools for TIL isolation and flow cytometry analysis
 
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In this webinar Dr. César Evaristo will describe best practices for isolation of tumor infiltrating leukocytes (TILs) and present an optimized workflow for faster and improved flow analysis. Webinar attendees will learn about innovative tools and solutions to increase quality of data, reduce experimental time, and ease scale-up during TIL analysis using flow cytometry. They will find out about optimized workflows for the isolation and flow cytometric phenotyping of mouse TILs, and methods to reduce biases during TIL analysis. Recent advances in immuno-oncology research have demonstrated the critical role of immune cells in cancer therapy. A deep understanding of the interactions and phenotypes of immune and tumor cells within the tumor microenvironment is therefore key to developing future immune-based cancer therapy strategies. However, dissociation of solid tumor samples prior to their use in downstream applications, such as immunomagnetic enrichment, flow cytometry, and single-cell sequencing, can be challenging. In addition, tumor cells often cause background signal and introduce bias to the analysis of immune cells, further complicating TIL research. Dr. César Evaristo, who received his doctorate from the University of Paris Descartes, will present the webinar. After seven years as a research scientist in the United States and Germany, he joined Miltenyi Biotec in 2016 as a team coordinator of the Tumor Immunology Group. He focuses on immuno-oncology preclinical research, target discovery, as well as oncology-related product development. Find more Miltenyi Biotec webinars at https://www.miltenyibiotec.com/macs-academy ABOUT MILTENYI BIOTEC Miltenyi Biotec is a global provider of products and services that advance biomedical research and cellular therapy. The company’s innovative tools support research at every level, from basic research to translational research to clinical application. This integrated portfolio enables scientists and clinicians to obtain, analyze, and utilize the cell. Miltenyi Biotec’s technologies cover techniques of sample preparation, cell isolation, cell sorting, flow cytometry, cell culture, molecular analysis, and preclinical imaging. Their more than 25 years of expertise spans research areas including immunology, stem cell biology, neuroscience, and cancer, and clinical research areas like hematology, graft engineering, and apheresis. In their commitment to the scientific community, Miltenyi Biotec also offers comprehensive scientific support, consultation, and expert training. Today, Miltenyi Biotec has more than 2,000 employees in 25 countries – all dedicated to helping researchers and clinicians around the world make a greater impact on science and health.
Views: 229 Miltenyi Biotec
Webinar: Sample Preparation for Flow Cytometry
 
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Join Dr. Frank Hardung and his webinar on sample preparation for flow cytometry – on tissue preparation, tissue dissociation, and sample clearance. Learn more at https://www.miltenyibiotec.com/macs-academy Miltenyi Biotec is a global provider of products and services that advance biomedical research and cellular therapy. Our innovative tools support research at every level, from basic research to translational research to clinical application. This integrated portfolio enables scientists and clinicians to obtain, analyze, and utilize the cell. Our technologies cover techniques of sample preparation, cell isolation, cell sorting, flow cytometry, cell culture, molecular analysis, and preclinical imaging. Our more than 25 years of expertise spans research areas including immunology, stem cell biology, neuroscience, and cancer, and clinical research areas like hematology, graft engineering, and apheresis. In our commitment to the scientific community, we also offer comprehensive scientific support, consultation, and expert training. Today, Miltenyi Biotec has more than 2,000 employees in 25 countries – all dedicated to helping researchers and clinicians around the world make a greater impact on science and health.
Views: 692 Miltenyi Biotec
Data analysis and applications of Flow Cytometry
 
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Subject:Biophysics Paper: Medical Physics
Views: 622 Vidya-mitra
Introduction to Flow Cytometry in R
 
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Instructor: Ryan Brinkman
Views: 1246 Bioinformatics DotCa
BD Accuri C6, flow cytometry made easy. Analyzing one cell at a time
 
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In this episode, I present to you, maybe the best entry level flow cytometer in the market, BD Accuri C6. I would say a must-have equipment for every lab focused on studying cells. Also, a brief introduction to flow cytometry and a step by step guide to measuring cell viability by this versatile technique.
Views: 778 Bio Sciences_4all
Webinar: Multicolor flow cytometry – understand the basics
 
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Join Dr Guerric Epron and his webinar on the basics of flow cytometry, including fluorescence, compensation, data-spread effect, and panel design. Find out more: https://www.miltenyibiotec.com/products/macs-flow-cytometry.html MACS Academy: https://www.miltenyibiotec.com/resources/macs-academy.html Miltenyi Biotec is a global provider of products and services that advance biomedical research and cellular therapy. Our innovative tools support research at every level, from basic research to translational research to clinical application. This integrated portfolio enables scientists and clinicians to obtain, analyze, and utilize the cell. Our technologies cover techniques of sample preparation, cell isolation, cell sorting, flow cytometry, cell culture, molecular analysis, and preclinical imaging. Our more than 25 years of expertise spans research areas including immunology, stem cell biology, neuroscience, and cancer, and clinical research areas like hematology, graft engineering, and apheresis. In our commitment to the scientific community, we also offer comprehensive scientific support, consultation, and expert training. Today, Miltenyi Biotec has more than 2,000 employees in 25 countries – all dedicated to helping researchers and clinicians around the world make a greater impact on science and health.
Views: 583 Miltenyi Biotec
Basics of flow cytometry, Part I:  Gating and data analysis
 
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This webinar covers the basic components of a flow cytometer, how to interpret a dye excitation/emission spectrum, how data is displayed, basic gating demonstration, and common statistics and terminology used in flow cytometry.
The Principle of Flow Cytometry and FACS (1- Flow Cytometry)
 
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This video is an explanation of Flow Cytometry, it contains a full explanation about flow cytometer and data obtaned from it.
Webinar: Analysis of Intracellular Signalling using Flow Cytometry
 
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Join Federica Villanova and her webinar on the analysis of intracellular signalling using flow cytometry. Learn more at http://www.miltenyibiotec.com/macs-academy Miltenyi Biotec is a global provider of products and services that advance biomedical research and cellular therapy. Our innovative tools support research at every level, from basic research to translational research to clinical application. This integrated portfolio enables scientists and clinicians to obtain, analyze, and utilize the cell. Our technologies cover techniques of sample preparation, cell isolation, cell sorting, flow cytometry, cell culture, molecular analysis, and preclinical imaging. Our more than 25 years of expertise spans research areas including immunology, stem cell biology, neuroscience, and cancer, and clinical research areas like hematology, graft engineering, and apheresis. In our commitment to the scientific community, we also offer comprehensive scientific support, consultation, and expert training. Today, Miltenyi Biotec has more than 2,000 employees in 25 countries – all dedicated to helping researchers and clinicians around the world make a greater impact on science and health.
Views: 1288 Miltenyi Biotec
Cell Cycle And Cell Cycle Assay
 
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Cell cycle is the most fundamental and important process by which eukaryotic cells duplicate and divide. This video will talk about the method to analysis the cell cycle.
Views: 301 Creative Bioarray
Trends in Multiparameter Flow Cytometry in Hematology - Dr. Brent Wood
 
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Flow cytometry is increasingly utilized as primary tool for the diagnosis of hematopoietic neoplasia in a clinical laboratory setting. The use of increased numbers of simultaneous fluorochromes offers the following potential advantages: increased accuracy of population identification, the ability to make better use of small specimens where cell number is a limiting factor, the processing of fewer tubes with the consequent reduction in reagents, technologist time and instrument time, the more efficient analysis of large numbers of cells which is important for the detection of small cell populations, and ultimately improved standardization. Brent Wood, associate professor of the hematology division in the department of laboratory medicine at the University of Washington discusses practical issues surrounding the implementation of high-level multicolor flow cytometry in a clinical laboratory setting and illustration of the power of the technology. Dr. Brent Wood
Views: 21157 UW Video
Webinar: Rare Cell Analysis in Flow Cytometry
 
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Join Frank Hardung and his webinar on rare cell analysis in flow cytometry, its general approaches, and proper controls. MACS Academy: https://www.miltenyibiotec.com/resources/macs-academy.html Miltenyi Biotec is a global provider of products and services that advance biomedical research and cellular therapy. Our innovative tools support research at every level, from basic research to translational research to clinical application. This integrated portfolio enables scientists and clinicians to obtain, analyze, and utilize the cell. Our technologies cover techniques of sample preparation, cell isolation, cell sorting, flow cytometry, cell culture, molecular analysis, and preclinical imaging. Our more than 25 years of expertise spans research areas including immunology, stem cell biology, neuroscience, and cancer, and clinical research areas like hematology, graft engineering, and apheresis. In our commitment to the scientific community, we also offer comprehensive scientific support, consultation, and expert training. Today, Miltenyi Biotec has more than 2,000 employees in 25 countries – all dedicated to helping researchers and clinicians around the world make a greater impact on science and health.
Views: 2180 Miltenyi Biotec
Multicolor Panel Building in Flow Cytometry
 
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For more information, visit https://www.bio-rad-antibodies.com/multicolor-webinar Multicolor flow cytometry is the analysis of multiple fluorescent parameters in one sample. Building large flow cytometry panels can be daunting because each additional fluorophore you add to your panel has the potential to influence another fluorophore. Join our webinar to learn which fluorophores are compatible with each other, how they interact and can affect your staining and how to obtain optimal resolution of signal. Furthermore, we will show you how dump channels, fluorophore brightness, antigen density, marker expression patterns and instrument configuration can all contribute to improving your panel design. This webinar is recommended for both novices and researchers with some experience of flow cytometry. Speaker Biography: Presented by: Dr Sebastian Hedlund, Flow Cytometry Specialist at Bio-Rad Laboratories Sebastian obtained his degree from the Institute of Technology, Linköping University, Sweden, followed by an MSc in Medical Biology from the same institute. He then moved to Freiburg, Germany where he obtained his PhD in Immunology and Molecular Biology at the Max Planck Institute of Immunobiology and Epigenetics. The focus of his thesis was the role of microRNA in innate immunity and immunopathology. He then left academia and started work as a dedicated flow cytometry specialist, before joining Bio-Rad in 2016 as the flow cytometry specialist for Scandinavia, Eastern Europe, Israel, and South Africa. Visit us on the web to learn more: https://www.bio-rad-antibodies.com/multicolor-panel-building-webinar.html We Are Bio-Rad. Our mission: To provide useful, high-quality products and services that advance scientific discovery and improve healthcare. At Bio-Rad, we are united behind this effort. These two objectives are the driving force behind every decision we make, from developing innovative ideas to building global solutions that help solve our customers' greatest challenges. Connect with Bio-Rad Online: Website: http://www.bio-rad.com/ LinkedIn: https://www.linkedin.com/company/1613226/ Facebook: https://www.facebook.com/biorad/ Twitter: https://twitter.com/BioRadLifeSci Instagram: @BioRadLabs Snapchat: @BioRadLabs
Views: 5153 Bio-Rad Laboratories
Flow cytometric analysis of mechanically-disaggregated bone marrow trephine biopsies
 
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This is a video to help show you the process of mechanically-disaggregating bone marrow trephine biopsies
Views: 124 Matt Wheeler
Flow Cytometry Webinar: From sample preparation to experimental design
 
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This advanced class in flow cytometry discusses sample preparation and the process of experimental design to ensure a successful flow cytometry experiment. Webinar topics include: 1. Cell source and preparation 2. Sample treatments 3. Controls and maximising signal 4. Choosing and using antibodies This video was produced by Innova Biosciences which is now fully integrated with Expedeon Ltd. - To find out more about our range of products for flow cytometry applications, please visit: https://bit.ly/2OiQEAs -To learn more about this powerful technique, feel free to download our guide: https://bit.ly/2IllhQo
Views: 47529 Expedeon
Fast Diagnosis of Antibiotic Resistance in Bacterial Infections Using Flow Cytometry
 
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http://www.thermofisher.com/us/en/home/life-science/cell-analysis/flow-cytometry/flow-cytometers/attune-acoustic-focusing-flow-cytometer.html?cid=BID_R01_PJT2395_999_VI_YUT_OD_KT_171110_TF_A_VD154 Kieran Mulroney, B.Sc., PhD candidate at the University of Western Australia, describes how the Invitrogen™ Attune™ NxT flow cytometer enables rapid and accurate profiling of the effect of drugs on individual bacterial cells.
Flow Cytometry - Double Stain
 
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( http://www.abnova.com ) - Step by step of double staining for flow cytometry analysis of cells is presented. Flow cytometry is a technique for measuring and examining microscopic particles in a flow system, which delivers particles singly by passing through an electronic detection apparatus. More videos at Abnova http://www.abnova.com
Views: 8517 Abnova
Advanced Topics in Cell Sorting
 
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For more info, visit http://www.bio-rad.com/yt/4/S3 In this webinar, Dan Fox of Propel Labs explores the principles of cell sorting including the theory of droplet cell sorting, how to assess cell sorter performance, and how sorting speed influences results. Dan also discusses the S3™ Cell Sorter’s unique sorting modes, the sort mode process and logic, and how the S3 Sorter simplifies the science of cell sorting. The first truly walk-up cell sorter, the S3 delivers an easy-to-use, affordable solution for busy scientists. The S3 Cell Sorter is an automated benchtop cell sorter featuring either one or two lasers and up to four fluorescence detectors plus forward- and side-scatter detectors. The S3 Cell Sorter is ideal for sorting cells expressing fluorescent proteins such as eGFP, DsRed, and mCherry or cells bearing fluorescent markers such as labeled antibodies. Features and Benefits • Simple instrument setup — automated drop delay calculation and droplet break-off monitoring enables precise 1–4 color sorting with minimal training • Compact footprint — only 70 × 65 × 65 cm (2.3 × 2.1 × 2.1 ft) including onboard fluidics and temperature control system • Reduced carryover — dual-position loading stage ensures the sample line remains clean between sorting runs • 2-way cell sorting — sort two different defined populations at the same time • High speed and high purity — sort cells fast while maintaining high sensitivity and purity • Intuitive interface — user-friendly ProSort™ software enables effortless instrument control and sort logic definition Presenter: Dan Fox Director of Research and Development Propel Labs, Fort Collins, Colorado Dan Fox has over a decade of experience in designing high-speed cell sorters, and holds numerous patents on flow cytometry and sorting technology. Dan is one of the principal designers who developed Bio-Rad’s S3 Cell Sorter. http://www.bio-rad.com/en-us/category/flow-cytometry?WT.mc_id=yt-GXD-ww-s3-20141006-N-kHOL6sJdM We Are Bio-Rad. Our mission: To provide useful, high-quality products and services that advance scientific discovery and improve healthcare. At Bio-Rad, we are united behind this effort. These two objectives are the driving force behind every decision we make, from developing innovative ideas to building global solutions that help solve our customers' greatest challenges. Connect with Bio-Rad Online: Website: http://www.bio-rad.com/ LinkedIn: https://www.linkedin.com/company/1613226/ Facebook: https://www.facebook.com/biorad/ Twitter: https://twitter.com/BioRadCellBio Instagram: @BioRadLabs Snapchat: @BioRadLabs
Views: 15592 Bio-Rad Laboratories
DNA Cell Cycle Analysis with Multicycle in FCS Express
 
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Learn how to use the Multicycle DNA Analysis tool in FCS Express as well as details on how modeling DNA data with Multicycle works. Kevin Becker, from Phoenix Flow Systems, discusses analysis of DNA Cell Cycle while the team at De Novo Software shows you how multicycle easily fits into your analysis routine directly in FCS Express. Often when trying to analyze DNA histograms for cell cycle or DNA analysis, the use of simple region markers on the histogram is not adequate. Sometimes it is necessary to use a powerful mathematical model to remove the debris component and deconvolute the multiple peaks in the histogram. The MultiCycle AV DNA analysis plug-in for FCS Express addresses this problem in a seamless manner. The mathematical model of Dean and Jett, the gold standard, is used by the MultiCycle AV plug-in. In addition, sophisticated debris subtraction models developed over the past decade by leaders in the field of cell cycle analysis, Rabinovitch & Bagwell, are applied to the debris component. This results in accurate, simple to understand cell cycle results from even the most complicated DNA histogram.
Views: 1046 DeNovoSoftware
Analysis of Cell Vitality and Viability Using Calcein-AM / PI
 
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Fluorescent Detection of Metabolically-Active and Non-viable Cells using Cellometer Image Cytometry
Views: 997 Nexcelom Bioscience
Benefits of recombinant antibody technology for flow cytometry
 
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Find out more on https://www.miltenyibiotec.com/reafinity Let Cap'n T Cell take you on a ride through the wonderful world of REAfinity™ Recombinant Antibodies. In this video, he will explain the benefits of using REAfinity Antibodies for flow cytometry experiments and will show you the features that make these antibodies so unique. REAfinity Antibodies are recombinantly generated antibodies that provide superior lot-to-lot consistency and purity, as compared to mouse or rat monoclonal antibodies. They have been engineered to lack background binding even without FcR blocking. In addition, they all have the same human IgG1 isotype, eliminating the need to include multiple isotype controls during flow analysis. Find out more on https://www.miltenyibiotec.com/reafinity Miltenyi Biotec is a global provider of products and services that advance biomedical research and cellular therapy. Our innovative tools support research at every level, from basic research to translational research to clinical application. This integrated portfolio enables scientists and clinicians to obtain, analyze, and utilize the cell. Our technologies cover techniques of sample preparation, cell isolation, cell sorting, flow cytometry, cell culture, molecular analysis, and preclinical imaging. Our more than 25 years of expertise spans research areas including immunology, stem cell biology, neuroscience, and cancer, and clinical research areas like hematology, graft engineering, and apheresis. In our commitment to the scientific community, we also offer comprehensive scientific support, consultation, and expert training. Today, Miltenyi Biotec has more than 2,000 employees in 25 countries - all dedicated to helping researchers and clinicians around the world make a greater impact on science and health.
Views: 380 Miltenyi Biotec
Combining RTCA With Flow Cytometry to Characterize BiTE/T Cell ...
 
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CYTO 2017 Workshop June 12, 2017 Workshop Title: Combining Real-Time Cell Analysis With Flow Cytometry to Characterize BiTE/T Cell-Mediated Cytolysis and Neutrophil Extracellular Traps Presenter: Brandon Lamarche, Ph.D., Sr. Scientist, ACEA Biosciences In this workshop you will learn about monitoring cellular impedance in real-time and without the use of labels. The xCELLigence instruments assess cell number, size, and attachment quality with unprecedented kinetic resolution and sensitivity. Herein we combine xCELLigence assays with NovoCyte flow cytometric analyses to characterize two key immunological prophylactic mechanisms. First, we track Bispecific T Cell Engager (BiTE)-mediated stimulation of cancer cell killing by T cells. Next, we shift our focus to NETosis, a cell death process that occurs when stimulated neutrophils cast an extracellular web containing DNA, histones, and anti-bacterial proteins that ensnare/neutralize invading organisms. Collectively, these talks will demonstrate how xCELLigence and flow assays can be run on the same sample to provide a wealth of information about cell health and behavior in diverse applications/contexts.

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